ArticlePloS one2011
Genome-wide DNA methylation analysis reveals phytoestrogen modification of promoter methylation patterns during embryonic stem cell differentiation.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Oral isoflavone supplementation on endometrial thickness: a meta-analysis of randomized placebo-controlled trials.Oncotarget · 2016Pooled it
- Transmission of an environmental modification in quails across three generations: changes in the contribution to phenotypic variability.Genetics, selection, evolution : GSE · 2026Article
- Article
- Skeletal Effects of Early-Life Exposure to Soy Isoflavones-A Review of Evidence From Rodent Models.Frontiers in pediatrics · 2020Review
- Early gestational maternal low-protein diet diminishes hepatic response to fasting in young adult male mice.Scientific reports · 2017Article
- Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles.BMC molecular biology · 2017Article
- Soy Formula and Epigenetic Modifications: Analysis of Vaginal Epithelial Cells from Infant Girls in the IFED Study.Environmental health perspectives · 2017Article
- Effect of dietary phytoestrogens on human growth regulation: imprinting in health & disease.The Indian journal of medical research · 2014Review
- Impact of soy isoflavones on the epigenome in cancer prevention.Nutrients · 2014Review
- Genistein exposure during the early postnatal period favors the development of obesity in female, but not male rats.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Environmental epigenetics and phytoestrogen/phytochemical exposures.The Journal of steroid biochemistry and molecular biology · 2014Review
- Long-term effects of chromatin remodeling and DNA damage in stem cells induced by environmental and dietary agents.Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnvironmental challenges during development affect the fetal epigenome, but the period(s) vulnerable to epigenetic dysregulation is(are) not clear. By employing a soy phytoestrogen, genistein, that is known to alter the epigenetic states of the A(vy) allele during embryogenesis, we have explored the sensitive period for epigenetic regulation. The post-implantation period, when de novo DNA methylation actively proceeds, is amenable to in vitro analysis using a mouse embryonic stem (ES) cell differentiation system. METHODS AND
findingsMouse ES cells were differentiated in the presence or absence of genistein, and DNA methylation patterns on day 10 were compared by microarray-based promoter methylation analysis coupled with a methylation-sensitive endonuclease (HpaII/McrBC)-dependent enrichment procedure. Moderate changes in methylation levels were observed in a subset of promoters following genistein treatment. Detailed investigation of the Ucp1 and Sytl1 promoters further revealed that genistein does not affect de novo methylation occurring between day 0 and day 4, but interferes with subsequent regulatory processes and leads to a decrease in methylation level for both promoters.
conclusionGenistein perturbed the methylation pattern of differentiated ES cells after de novo methylation. Our observations suggest that, for a subset of genes, regulation after de novo DNA methylation in the early embryo may be sensitive to genistein.
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