ArticlePloS one2011
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
73 citing papers in PubMed, 221 citations in OpenAlex.
- Sediments as Potential Sources of Non-Cyanobacterial Diazotrophs in Arctic Sea Ice and Seawater.Environmental microbiology reports · 2026Article
- Highly diverse diazotrophs drive high NFundamental research · 2026Article
- A genomic view of Earth's biomes.Nature reviews. Genetics · 2026Review
- Contrasting Nitrogen Fixation Between Arctic and Atlantic Waters in the Fram Strait.Microbial ecology · 2025Article
- Exploring the interactions between algae and archaea.Marine life science & technology · 2025Review
- Non-cyanobacterial diazotrophs support the survival of marine microalgae in nitrogen-depleted environment.Genome biology · 2025Article
- Unveiling the contribution of particle-associated non-cyanobacterial diazotrophs to NCommunications biology · 2025Article
- Particle-associated NScience advances · 2025Article
- Organic metabolite uptake by diazotrophs in the North Pacific Ocean.ISME communications · 2025Article
- Chemoautotrophic Thermodesulfobacteriota as a key genomic potential group in the hypoxic diazotrophic community of the Changjiang (Yangtze River) estuary.Frontiers in microbiology · 2025Article
- Diverse community of rhizobia-diatom symbioses fixes nitrogen in the South Pacific gyre.ISME communications · 2025Article
- Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotrophScience advances · 2024Article
- Article
- Marine nitrogen-fixers in the Canadian Arctic Gateway are dominated by biogeographically distinct noncyanobacterial communities.FEMS microbiology ecology · 2023Article
- Non-cyanobacterial diazotrophs: global diversity, distribution, ecophysiology, and activity in marine waters.FEMS microbiology reviews · 2023Review
- Stochastic Processes Dominate in the Water Mass-Based Segregation of Diazotrophs in a High Arctic Fjord (Svalbard).Microbial ecology · 2023Article
- Hydrodynamic regimes modulate nitrogen fixation and the mode of diazotrophy in Lake Tanganyika.Nature communications · 2023Article
- Genetic and physiological insights into the diazotrophic activity of a non-cyanobacterial marine diazotroph.Environmental microbiology · 2022Article
- Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre.Nature communications · 2022Article
- Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for NThe ISME journal · 2022Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are thought to be the main N(2)-fixing organisms (diazotrophs) in marine pelagic waters, but recent molecular analyses indicate that non-cyanobacterial diazotrophs are also present and active. Existing data are, however, restricted geographically and by limited sequencing depths. Our analysis of 79,090 nitrogenase (nifH) PCR amplicons encoding 7,468 unique proteins from surface samples (ten DNA samples and two RNA samples) collected at ten marine locations world-wide provides the first in-depth survey of a functional bacterial gene and yield insights into the composition and diversity of the nifH gene pool in marine waters. Great divergence in nifH composition was observed between sites. Cyanobacteria-like genes were most frequent among amplicons from the warmest waters, but overall the data set was dominated by nifH sequences most closely related to non-cyanobacteria. Clusters related to Alpha-, Beta-, Gamma-, and Delta-Proteobacteria were most common and showed distinct geographic distributions. Sequences related to anaerobic bacteria (nifH Cluster III) were generally rare, but preponderant in cold waters, especially in the Arctic. Although the two transcript samples were dominated by unicellular cyanobacteria, 42% of the identified non-cyanobacterial nifH clusters from the corresponding DNA samples were also detected in cDNA. The study indicates that non-cyanobacteria account for a substantial part of the nifH gene pool in marine surface waters and that these genes are at least occasionally expressed. The contribution of non-cyanobacterial diazotrophs to the global N(2) fixation budget cannot be inferred from sequence data alone, but the prevalence of non-cyanobacterial nifH genes and transcripts suggest that these bacteria are ecologically significant.
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