ArticlePloS one2011
Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Dietary supplementation with methionine and/or turmeric oil enhances carcass cuts, economics, meat quality, and antioxidant status in broilers: insights into DNA methyltransferase transcripts.Tropical animal health and production · 2026Article
- A methyl donor and an essential oil: Performance, economic efficiency, serum biochemistry, histomorphometry, and appetite/growth-related gene expression in broilers.Veterinary research communications · 2026Article
- Reinforcement of repressive marks in the chicken primordial germ cell epigenetic signature: divergence from basal state resetting in mammals.Epigenetics & chromatin · 2024Article
- Review
- Female Germ Cell Development in Chickens and Humans: The Chicken Oocyte Enriched Genes Convergent and Divergent with the Human Oocyte.International journal of molecular sciences · 2022Review
- Role of gga-miR-29b-3p in suppressing the proliferation, invasion and migration of MSB1 Marek's disease tumor cells by the targeting of theAnnals of translational medicine · 2022Article
- Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens.Journal of animal science and biotechnology · 2022Article
- Finer resolution analysis of transcriptional programming during the active migration of chicken primordial germ cells.Computational and structural biotechnology journal · 2022Article
- Dissecting chicken germ cell dynamics by combining a germ cell tracing transgenic chicken model with single-cell RNA sequencing.Computational and structural biotechnology journal · 2022Article
- A Concise Review of MicroRNA-383: Exploring the Insights of Its Function in Tumorigenesis.Journal of Cancer · 2022Review
- Effect of miR-302b MicroRNA Inhibition on Chicken Primordial Germ Cell Proliferation and Apoptosis Rate.Genes · 2021Article
- Zygotic genome activation in the chicken: a comparative review.Cellular and molecular life sciences : CMLS · 2020Review
- Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen.Frontiers in veterinary science · 2020Article
- The Importance of Small Non-Coding RNAs in Human Reproduction: A Review Article.The application of clinical genetics · 2020Review
- Transgenerational epigenetic inheritance in birds.Environmental epigenetics · 2018Review
- A NovelFrontiers in physiology · 2018Article
- Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model.Frontiers in genetics · 2018Review
- miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.Cell death & disease · 2017Article
- Dynamics of miRNA transcriptome during gonadal development of zebrafish.Scientific reports · 2017Article
- MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans.Stem cells international · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is widespread in most species, from bacteria to mammals, and is crucial for genomic imprinting, gene expression, and embryogenesis. DNA methylation occurs via two major classes of enzymatic reactions: maintenance-type methylation catalyzed by DNA (cytosine-5-)-methyltransferase (DNMT) 1, and de novo methylation catalyzed by DNMT 3 alpha (DNMT3A) and -beta (DNMT3B). The expression pattern and regulation of DNMT genes in primordial germ cells (PGCs) and germ line cells has not been sufficiently established in birds. Therefore, we employed bioinformatics, RT-PCR, real-time PCR, and in situ hybridization analyses to examine the structural conservation and conserved expression patterns of chicken DNMT family genes. We further examined the regulation of a candidate de novo DNA methyltransferase gene, cDNMT3B by cotransfection of cDNMT3B 3'UTR- and cDNMT3B 3'UTR-specific miRNAs through a dual fluorescence reporter assay. All cDNMT family members were differentially detected during early embryonic development. Of interest, cDNMT3B expression was highly detected in early embryos and in PGCs. During germ line development and sexual maturation, cDNMT3B expression was reestablished in a female germ cell-specific manner. In the dual fluorescence reporter assay, cDNMT3B expression was significantly downregulated by four miRNAs: gga-miR-15c (25.82%), gga-miR-29b (30.01%), gga-miR-383 (30.0%), and gga-miR-222 (31.28%). Our data highlight the structural conservation and conserved expression patterns of chicken DNMTs. The miRNAs investigated in this study may induce downregulation of gene expression in chicken PGCs and germ cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.