ArticlePloS one2011
Proteomic analyses reveal common promiscuous patterns of cell surface proteins on human embryonic stem cells and sperms.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 44 citations in OpenAlex.
- Quantitative Proteomic Analysis of APP/PS1 Transgenic Mice.Current Alzheimer research · 2026Article
- Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry andAnimal biotechnology · 2024Article
- NaInternational journal of molecular sciences · 2023Review
- Control of intracellular pH and bicarbonate by CONature communications · 2023Article
- Using publicly available transcriptomic data to identify mechanistic and diagnostic biomarkers in azoospermia and overall male infertility.Scientific reports · 2022Article
- Generating aptamers towards human sperm cells using massively parallel sequencing.Analytical and bioanalytical chemistry · 2021Article
- The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021Review
- Comparative Proteomics Analysis of Four Commonly Used Methods for Identification of Novel Plasma Membrane Proteins.The Journal of membrane biology · 2019Article
- Genomic study and Medical Subject Headings enrichment analysis of early pregnancy rate and antral follicle numbers in Nelore heifers.Journal of animal science · 2017Article
- Characterization of the functions and proteomes associated with membrane rafts in chicken sperm.PloS one · 2017Article
- Article
- Epigenetic regulation of open chromatin in pluripotent stem cells.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Cell surface proteomics analysis indicates a neural lineage bias of rat bone marrow mesenchymal stromal cells.BioMed research international · 2014Article
- Molecular mechanisms controlling the cell cycle in embryonic stem cells.Stem cell reviews and reports · 2013Review
- Plasma membrane proteomics of tumor spheres identify CD166 as a novel marker for cancer stem-like cells in head and neck squamous cell carcinoma.Molecular & cellular proteomics : MCP · 2013Article
- Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal.Stem cells and development · 2013Article
- Human sperm tail proteome suggests new endogenous metabolic pathways.Molecular & cellular proteomics : MCP · 2013Article
- Aire promotes the self-renewal of embryonic stem cells through Lin28.Stem cells and development · 2012Article
- The Use of Proteomics in Assisted Reproduction.In vivo (Athens, Greece)Review
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Authors and funding
10 authors at 6 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has long been proposed that early embryos and reproductive organs exhibit similar gene expression profiles. However, whether this similarity is propagated to the protein level remains largely unknown. We have previously characterised the promiscuous expression pattern of cell surface proteins on mouse embryonic stem (mES) cells. As cell surface proteins also play critical functions in human embryonic stem (hES) cells and germ cells, it is important to reveal whether a promiscuous pattern of cell surface proteins also exists for these cells. METHODS AND PRINCIPAL
findingsSurface proteins of hES cells and human mature sperms (hSperms) were purified by biotin labelling and subjected to proteomic analyses. More than 1000 transmembrane or secreted cell surface proteins were identified on the two cell types, respectively. Proteins from both cell types covered a large variety of functional categories including signal transduction, adhesion and transporting. Moreover, both cell types promiscuously expressed a wide variety of tissue specific surface proteins, and some surface proteins were heterogeneously expressed. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that the promiscuous expression of functional and tissue specific cell surface proteins may be a common pattern in embryonic stem cells and germ cells. The conservation of gene expression patterns between early embryonic cells and reproductive cells is propagated to the protein level. These results have deep implications for the cell surface signature characterisation of pluripotent stem cells and germ cells and may lead the way to a new area of study, i.e., the functional significance of promiscuous gene expression in pluripotent and germ cells.
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