ArticlePloS one2011
Universal oligonucleotide microarray for sub-typing of Influenza A virus.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- The peculiar characteristics and advancement in diagnostic methodologies of influenza A virus.Frontiers in microbiology · 2024Review
- Review
- A computational toolset for rapid identification of SARS-CoV-2, other viruses and microorganisms from sequencing data.Briefings in bioinformatics · 2021Article
- FluChip-8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay.Influenza and other respiratory viruses · 2020Article
- Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.Journal of virological methods · 2019Article
- A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection.Journal of visualized experiments : JoVE · 2018Article
- Using NMR and molecular dynamics to link structure and dynamics effects of the universal base 8-aza, 7-deaza, N8 linked adenosine analog.Nucleic acids research · 2016Article
- Highly Pathogenic Avian Influenza Virus A/H5N1 Infection in Vaccinated Meat Duck Flocks in the Mekong Delta of Vietnam.Transboundary and emerging diseases · 2016Article
- Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray.SpringerPlus · 2016Article
- High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.PloS one · 2016Article
- Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections--needs, advances, and future prospects.The Lancet. Infectious diseases · 2014Review
- A DNA microarray-based assay to detect dual infection with two dengue virus serotypes.Sensors (Basel, Switzerland) · 2014Article
- Design of a set of probes with high potential for influenza virus epidemiological surveillance.Bioinformation · 2013Article
- Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9). Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps. Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36 unambiguously identified HA and NA subtypes of Influenza A virus.
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