ArticleJournal of visualized experiments : JoVE2011
Using a pan-viral microarray assay (Virochip) to screen clinical samples for viral pathogens.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 84 citations in OpenAlex.
- Beyond the Gut: The intratumoral microbiome's influence on tumorigenesis and treatment response.Cancer communications (London, England) · 2024Review
- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Screen the unforeseen: Microbiome-profiling for detection of zoonotic pathogens in wild rats.Transboundary and emerging diseases · 2022Article
- Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis.Scientific reports · 2022Article
- Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray.Frontiers in microbiology · 2021Article
- Genomics-based re-examination of the taxonomy and phylogeny of human and simian Mastadenoviruses: an evolving whole genomes approach, revealing putative zoonosis, anthroponosis, and amphizoonosis.Cladistics : the international journal of the Willi Hennig Society · 2020Article
- A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents.Journal of translational medicine · 2019Article
- Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients.BioMed research international · 2019Article
- Identification of a putative novel genotype 3/rabbit hepatitis E virus (HEV) recombinant.PloS one · 2018Article
- No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs.BMC veterinary research · 2017Article
- The Human Virome: Implications for Clinical Practice in Transplantation Medicine.Journal of clinical microbiology · 2017Review
- The eukaryotic gut virome in hematopoietic stem cell transplantation: new clues in enteric graft-versus-host disease.Nature medicine · 2017Article
- Detection of Peste Des Petits Ruminants Virus (PPRV) Genome from Nasal Swabs of Dogs.Current microbiology · 2016Article
- Enhanced virome sequencing using targeted sequence capture.Genome research · 2015Article
- Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis.Genome medicine · 2015Article
- Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification.Journal of virological methods · 2015Article
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- DNA microarray for detection of gastrointestinal viruses.Journal of clinical microbiology · 2015Article
- Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection.Journal of virological methods · 2014Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The diagnosis of viral causes of many infectious diseases is difficult due to the inherent sequence diversity of viruses as well as the ongoing emergence of novel viral pathogens, such as SARS coronavirus and 2009 pandemic H1N1 influenza virus, that are not detectable by traditional methods. To address these challenges, we have previously developed and validated a pan-viral microarray platform called the Virochip with the capacity to detect all known viruses as well as novel variants on the basis of conserved sequence homology. Using the Virochip, we have identified the full spectrum of viruses associated with respiratory infections, including cases of unexplained critical illness in hospitalized patients, with a sensitivity equivalent to or superior to conventional clinical testing. The Virochip has also been used to identify novel viruses, including the SARS coronavirus, a novel rhinovirus clade, XMRV (a retrovirus linked to prostate cancer), avian bornavirus (the cause of a wasting disease in parrots), and a novel cardiovirus in children with respiratory and diarrheal illness. The current version of the Virochip has been ported to an Agilent microarray platform and consists of ~36,000 probes derived from over ~1,500 viruses in GenBank as of December of 2009. Here we demonstrate the steps involved in processing a Virochip assay from start to finish (~24 hour turnaround time), including sample nucleic acid extraction, PCR amplification using random primers, fluorescent dye incorporation, and microarray hybridization, scanning, and analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.