ArticleDevelopmental neurobiology2011
The actin nucleating Arp2/3 complex contributes to the formation of axonal filopodia and branches through the regulation of actin patch precursors to filopodia.
Article in Developmental neurobiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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78 citing papers in PubMed, 124 citations in OpenAlex.
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- Honeycomb Biosilica in Sponges: From Understanding Principles of Unique Hierarchical Organization to Assessing Biomimetic Potential.Biomimetics (Basel, Switzerland) · 2023Article
- Interneuron odyssey: molecular mechanisms of tangential migration.Frontiers in neural circuits · 2023Review
- Activating RAC1 variants in the switch II region cause a developmental syndrome and alter neuronal morphology.Brain : a journal of neurology · 2022Article
- Antagonistic Activities of Fmn2 and ADF Regulate Axonal F-Actin Patch Dynamics and the Initiation of Collateral Branching.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- The Role of Spastin in Axon Biology.Frontiers in cell and developmental biology · 2022Review
- SARM1 Suppresses Axon Branching Through Attenuation of Axonal Cytoskeletal Dynamics.Frontiers in molecular neuroscience · 2022Article
- Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.eLife · 2021Article
- A two-step actin polymerization mechanism drives dendrite branching.Neural development · 2021Article
- Cytoskeleton and Membrane Organization at Axon Branches.Frontiers in cell and developmental biology · 2021Review
- Intra-axonal mechanisms driving axon regeneration.Brain research · 2020Review
- Linking Alpha-Synuclein to the Actin Cytoskeleton: Consequences to Neuronal Function.Frontiers in cell and developmental biology · 2020Review
- Axonal cytomechanics in neuronal development.Journal of biosciences · 2020Review
18 more citing papers are in PubMed but not listed here.
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8 authors at 3 institutions in 1 country.
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Abstract
The emergence of axonal filopodia is the first step in the formation of axon collateral branches. In vitro, axonal filopodia emerge from precursor cytoskeletal structures termed actin patches. However, nothing is known about the cytoskeletal dynamics of the axon leading to the formation of filopodia in the relevant tissue environment. In this study we investigated the role of the actin nucleating Arp2/3 complex in the formation of sensory axon actin patches, filopodia, and branches. By combining in ovo chicken embryo electroporation mediated gene delivery with a novel acute ex vivo spinal cord preparation, we demonstrate that actin patches form along sensory axons and give rise to filopodia in situ. Inhibition of Arp2/3 complex function in vitro and in vivo decreases the number of axonal filopodia. In vitro, Arp2/3 complex subunits and upstream regulators localize to actin patches. Analysis of the organization of actin filaments in actin patches using platinum replica electron microscopy reveals that patches consist of networks of actin filaments, and filaments in axonal filopodia exhibit an organization consistent with the Arp2/3-based convergent elongation mechanism. Nerve growth factor (NGF) promotes formation of axonal filopodia and branches through phosphoinositide 3-kinase (PI3K). Inhibition of the Arp2/3 complex impairs NGF/PI3K-induced formation of axonal actin patches, filopodia, and the formation of collateral branches. Collectively, these data reveal that the Arp2/3 complex contributes to the formation of axon collateral branches through its involvement in the formation of actin patches leading to the emergence of axonal filopodia.
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