Evidence map›Paper›PMID 21557223›Full record

ArticleJournal of pharmaceutical sciences2011

Effect of benzyl alcohol on recombinant human interleukin-1 receptor antagonist structure and hydrogen-deuterium exchange.

John R Alford, Andrew C Fowler, Deborah S Wuttke, Bruce A Kerwin, Ramil F Latypov, John F Carpenter, Theodore W Randolph

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

John R AlfordDepartment of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309.
Andrew C Fowler
Deborah S Wuttke
Bruce A Kerwin
Ramil F Latypov
John F Carpenter
Theodore W Randolph

Funding

Biotechnology Research Partnerships Renewal: Aggregation of Therapeutic ProteinsR01EB006006 · NIBIB · UNIVERSITY OF COLORADO · PI RANDOLPH, THEODORE W · 2006 to 2017
$7.2M
Purchase of 900 MHz NMR SpectrometerP41GM068928 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI WUTTKE, DEBORAH S. · 2003 to 2009
$6.4M
SINGLE STRANDED DNA RECOGNITION IN TELOMERESR01GM059414 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI WUTTKE, DEBORAH S. · 1999 to 2015
$3.7M
NIBIB NIH HHS 1 R01 EB006006-01NIBIB NIH HHS R01 EB006006NIGMS NIH HHS P41 GM068928NIGMS NIH HHS R01 GM059414
6 · The paper itself

Abstract

Benzyl alcohol, a preservative commonly added to multidose therapeutic protein formulations, can accelerate aggregation of recombinant human interleukin-1 receptor antagonist (rhIL-1ra). To investigate the interactions between benzyl alcohol and rhIL-1ra, we used nuclear magnetic resonance to observe the effect of benzyl alcohol on the chemical shifts of amide resonances of rhIL-1ra and to measure hydrogen-deuterium exchange rates of individual rhIL-1ra residues. Addition of 0.9% benzyl alcohol caused significant chemical shifts of amide resonances for residues 90-97, suggesting that these solvent-exposed residues participate in the binding of benzyl alcohol. In contrast, little perturbation of exchange rates was observed in the presence of either sucrose or benzyl alcohol.

Indexed as

Deuterium Exchange MeasurementBenzyl AlcoholBinding SitesChemistry, PharmaceuticalHumansInterleukin 1 Receptor Antagonist ProteinMagnetic Resonance SpectroscopyModels, MolecularPreservatives, PharmaceuticalProtein AggregatesProtein BindingProtein ConformationRecombinant ProteinsStructure-Activity RelationshipTechnology, PharmaceuticalTemperatureBenzyl AlcoholIL1RN protein, humanInterleukin 1 Receptor Antagonist ProteinPreservatives, PharmaceuticalProtein AggregatesRecombinant ProteinsExcipientsNMR spectroscopyProtein aggregationProtein formulationProtein structure

Identifiers

PMID21557223
PMCPMC6261337

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.