ArticleProceedings of the National Academy of Sciences of the United States of America2011
Solute diffusion is hindered in the mitochondrial matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Carbon-to-ATP ratios across the kingdoms of life.iScience · 2026Article
- Diffusive spreading across dynamic mitochondrial network architectures.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Organelles harbour pH gradients.Research square · 2026Article
- Organelles harbour pH gradients.bioRxiv : the preprint server for biology · 2025Article
- Protein misfolding involving entanglements providesa structural explanation for the origin of stretched-exponential refolding kinetics.Science advances · 2025Article
- Endoplasmic reticulum network heterogeneity guides diffusive transport and kinetics.Biophysical journal · 2023Article
- Stress-dependent macromolecular crowding in the mitochondrial matrix.The EMBO journal · 2023Article
- Single-Molecule Displacement Mapping Unveils Sign-Asymmetric Protein Charge Effects on Intraorganellar Diffusion.Nano letters · 2023Article
- Caveolin-1 controls mitochondrial damage and ROS production by regulating fission - fusion dynamics and mitophagy.Redox biology · 2022Article
- Investigation of apoptosis based on fluorescence lifetime imaging microscopy with a mitochondria-targeted viscosity probe.RSC advances · 2021Article
- Microvesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells.Journal of controlled release : official journal of the Controlled Release Society · 2021Article
- Superoxide free radical spin-lattice relaxivity: A quench-assisted MR study.Magnetic resonance in medicine · 2021Article
- Modeling the Effects of Calcium Overload on Mitochondrial Ultrastructural Remodeling.Applied sciences (Basel, Switzerland) · 2021Article
- Intracellular quality control of mitochondrial DNA: evidence and limitations.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Mitochondrial morphology provides a mechanism for energy buffering at synapses.Scientific reports · 2019Article
- Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System.ACS chemical biology · 2019Article
- Mitochondrial Morphofunction in Mammalian Cells.Antioxidants & redox signaling · 2019Review
- Consequences of Heterogeneous Crowding on an Enzymatic Reaction: A Residence Time Monte Carlo Approach.ACS omega · 2019Article
- Targeted fluorescence lifetime probes reveal responsive organelle viscosity and membrane fluidity.PloS one · 2019Article
- Insights on the impact of mitochondrial organisation on bioenergetics in high-resolution computational models of cardiac cell architecture.PLoS computational biology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular chemical reactions generally constitute reaction-diffusion systems located inside nanostructured compartments like the cytosol, nucleus, endoplasmic reticulum, Golgi, and mitochondrion. Understanding the properties of such systems requires quantitative information about solute diffusion. Here we present a novel approach that allows determination of the solvent-dependent solute diffusion constant (D(solvent)) inside cell compartments with an experimentally quantifiable nanostructure. In essence, our method consists of the matching of synthetic fluorescence recovery after photobleaching (FRAP) curves, generated by a mathematical model with a realistic nanostructure, and experimental FRAP data. As a proof of principle, we assessed D(solvent) of a monomeric fluorescent protein (AcGFP1) and its tandem fusion (AcGFP1(2)) in the mitochondrial matrix of HEK293 cells. Our results demonstrate that diffusion of both proteins is substantially slowed by barriers in the mitochondrial matrix (cristae), suggesting that cells can control the dynamics of biochemical reactions in this compartment by modifying its nanostructure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.