ArticleThe Biochemical journal2011
Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed, 180 citations in OpenAlex.
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- A Randomized Trial of the Effects of Dietary n3-PUFAs on Skeletal Muscle Function and Acute Exercise Response in Healthy Older Adults.Nutrients · 2022Trial
- Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males.Physiological reports · 2018Trial
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- Preserved mitochondrial respiration in presence of oxidative stress and reduced mitochondrial mass after 10-day bed rest in older adults.The Journal of physiology · 2026Article
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- BCL6 regulates skeletal muscle mass and mitochondrial bioenergetics.Molecular metabolism · 2026Article
- One half of the sprinting coin: the oxygen transport cascade of a 91-yr-old female world-record sprinter.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Remodeling of the Mouse Liver and Skeletal Muscle Metabolome in Response to Continuous Acute Exercise and Disruption of AMPK-Glycogen Interactions.Metabolites · 2026Article
- Dietary nitrate supplementation mitigates age-related changes at the neuromuscular junction in mice.The Journal of physiology · 2026Article
- Quantification of Mitochondrial Oxidative Phosphorylation in Skeletal Muscle.Methods in molecular biology (Clifton, N.J.) · 2026Article
- PTEN regulates vagal-insulin signaling to optimize autonomic output determining peripheral inflammatory and metabolic homeostasis.Nature communications · 2025Article
- Biological sex does not impact intrinsic mitochondrial respiration supported by complexes I and II in human skeletal muscle.Experimental physiology · 2025Article
- Mitochondrial-targeted plastoquinone therapy prevents early onset muscle weakness that occurs before atrophy during ovarian cancer.Molecular metabolism · 2025Article
- Evolutionary diversity of muscle OXPHOS efficiencyProceedings. Biological sciences · 2025Article
- Mitochondrial Sensitivity to Submaximal [ADP] Following Bed Rest: A Novel Two-Phase Approach Associated With Fibre Types.Journal of cachexia, sarcopenia and muscle · 2025Article
- Multiomic analysis of different horse breeds reveals that gut microbial butyrate enhances racehorse athletic performance.NPJ biofilms and microbiomes · 2025Article
- Semaglutide-induced weight loss improves mitochondrial energy efficiency in skeletal muscle.Obesity (Silver Spring, Md.) · 2025Article
- Platelet bioenergetics correlate with skeletal muscle respiration in a murine model of type II diabetes.Frontiers in molecular biosciences · 2025Article
54 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Assessment of mitochondrial ADP-stimulated respiratory kinetics in PmFBs (permeabilized fibre bundles) is increasingly used in clinical diagnostic and basic research settings. However, estimates of the Km for ADP vary considerably (~20-300 μM) and tend to overestimate respiration at rest. Noting that PmFBs spontaneously contract during respiration experiments, we systematically determined the impact of contraction, temperature and oxygenation on ADP-stimulated respiratory kinetics. BLEB (blebbistatin), a myosin II ATPase inhibitor, blocked contraction under all conditions and yielded high Km values for ADP of >~250 and ~80 μM in red and white rat PmFBs respectively. In the absence of BLEB, PmFBs contracted and the Km for ADP decreased ~2-10-fold in a temperature-dependent manner. PmFBs were sensitive to hyperoxia (increased Km) in the absence of BLEB (contracted) at 30 °C but not 37 °C. In PmFBs from humans, contraction elicited high sensitivity to ADP (Km<100 μM), whereas blocking contraction (+BLEB) and including a phosphocreatine/creatine ratio of 2:1 to mimic the resting energetic state yielded a Km for ADP of ~1560 μM, consistent with estimates of in vivo resting respiratory rates of <1% maximum. These results demonstrate that the sensitivity of muscle to ADP varies over a wide range in relation to contractile state and cellular energy charge, providing evidence that enzymatic coupling of energy transfer within skeletal muscle becomes more efficient in the working state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.