Evidence map›Paper›PMID 21553893›Full record

ArticleBioconjugate chemistry2011

Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging.

Gopal Iyer, Fabien Pinaud, Jianmin Xu, Yuval Ebenstein, Jack Li, Jessica Chang, Maxime Dahan, Shimon Weiss

Abstract read
In one paragraph

Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Production and targeting of monovalent quantum dots.Journal of visualized experiments : JoVE · 2014
    Article
  6. A genetically encoded aldehyde for rapid protein labelling.Chemical communications (Cambridge, England) · 2014
    Article
  7. Article
  8. Labeling Cytosolic Targets in Live Cells with Blinking Probes.The journal of physical chemistry letters · 2013
    Article
  9. Article
  10. Review
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Gopal IyerDepartment of Chemistry and Biochemistry, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095, United States. gopal@chem.ucla.edu
Fabien Pinaud
Jianmin Xu
Yuval Ebenstein
Jack Li
Jessica Chang
Maxime Dahan
Shimon Weiss
University of California, Los Angeles · USCalifornia NanoSystems Institute · USÉcole Normale Supérieure - PSL · FRLaboratoire Kastler Brossel · FR

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KITCHEN, SCOTT G · 1991 to 2018
$39.9M
Multimodal Qdot Probes for Bioimaging of Cells and Tumors in Small AnimalsR01EB000312 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2002 to 2010
$7.5M
NCI NIH HHS CA-16042NCI NIH HHS P30 CA016042NIAID NIH HHS AI-28697NIAID NIH HHS P30 AI028697NIBIB NIH HHS R01 EB000312NIBIB NIH HHS R01-EB000312
6 · The paper itself

Abstract

We present a robust scheme for preparation of semiconductor quantum dots (QDs) and cognate partners in a conjugation ready format. Our approach is based on bis-aryl hydrazone bond formation mediated by aromatic aldehyde and hydrazinonicotinate acetone hydrazone (HyNic) activated peptide coated quantum dots. We demonstrate controlled preparation of antibody--QD bioconjugates for specific targeting of endogenous epidermal growth factor receptors in breast cancer cells and for single QD tracking of transmembrane proteins via an extracellular epitope. The same approach was also used for optical mapping of RNA polymerases bound to combed genomic DNA in vitro.

Indexed as

Quantum DotsAldehydesAnimalsAntibodiesCell Line, TumorCell SurvivalCHO CellsCoated Materials, BiocompatibleCricetinaeCricetulusErbB ReceptorsFemaleHeLa CellsHumansHydrazinesMaterials TestingAldehydesAntibodiesCoated Materials, BiocompatibleErbB ReceptorsHydrazinesPeptides

Identifiers

PMID21553893
PMCPMC3116077
OpenAlexW1982789768

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.