ArticleCurrent protocols in toxicology2011
Cellular fura-2 manganese extraction assay (CFMEA).
Article in Current protocols in toxicology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 20 citations in OpenAlex.
- A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters.Bio-protocol · 2026Article
- Prolonged duration induces divergent transcriptomic responses to manganese, distinct from concentration effects, in an SH-SY5Y neurotoxicity model.Neurotoxicology · 2026Article
- Molecular mechanisms of SLC30A10-mediated manganese transport.Nature communications · 2025Article
- IGF/mTORC1/S6 Signaling Is Potentiated and Prolonged by Acute Loading of Subtoxicological Manganese Ion.Biomolecules · 2023Article
- Insights into the regulation of cellular MnBiochimica et biophysica acta. Molecular basis of disease · 2023Article
- Impaired XK recycling for importing manganese underlies striatal vulnerability in Huntington's disease.The Journal of cell biology · 2022Article
- Identification of Three Small Molecules That Can Selectively Influence Cellular Manganese Levels in a Mouse Striatal Cell Model.Molecules (Basel, Switzerland) · 2021Article
- Manganese-induced Mitochondrial Dysfunction Is Not Detectable at Exposures Below the Acute Cytotoxic Threshold in Neuronal Cell Types.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese.The Journal of biological chemistry · 2020Article
- Huntington's disease associated resistance to Mn neurotoxicity is neurodevelopmental stage and neuronal lineage dependent.Neurotoxicology · 2019Article
- Zinc transporter 10 (ZnT10)-dependent extrusion of cellular MnThe Journal of biological chemistry · 2019Article
- Reduced membrane cholesterol after chronic hypoxia limits Orai1-mediated pulmonary endothelial CaAmerican journal of physiology. Heart and circulatory physiology · 2018Article
- Microglia amplify inflammatory activation of astrocytes in manganese neurotoxicity.Journal of neuroinflammation · 2017Article
- Redox dynamics of manganese as a mitochondrial life-death switch.Biochemical and biophysical research communications · 2017Review
- Manganese-Induced Parkinsonism and Parkinson's Disease: Shared and Distinguishable Features.International journal of environmental research and public health · 2015Review
- Metals, oxidative stress and neurodegeneration: a focus on iron, manganese and mercury.Neurochemistry international · 2013Review
- Optimization of fluorescence assay of cellular manganese status for high throughput screening.Journal of biochemical and molecular toxicology · 2013Article
- DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction.ACS chemical neuroscience · 2012Article
- Role of manganese in neurodegenerative diseases.Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura-2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the method is equally adaptable to other cultured mammalian cells. An ultrasensitive fluorescent nucleic acid stain for quantification of double-stranded DNA (dsDNA) in solution, Quant-iT PicoGreen, has been utilized for normalization of Mn concentration in the cultured cells, following Mn (II) chloride (MnCl(2)) exposure. Depending on the cell type and density, other methods, e.g., protein determination assays or cell counts, may also be used for normalization. Methods are described for rapidly stopping Mn uptake and transport processes at specified times, extraction, and quantification of cellular Mn content, and normalization of Mn levels to dsDNA concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.