ArticleThe journal of physical chemistry. C, Nanomaterials and interfaces2011
Metallic-Nanostructure-Enhanced Fluorescence of Single Flavin Cofactor and Single Flavoenzyme Molecules.
Article in The journal of physical chemistry. C, Nanomaterials and interfaces, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Fluorescence Intensity and Lifetime Cell Imaging with Luminescent Gold Nanoclusters.The journal of physical chemistry. C, Nanomaterials and interfaces · 2012Article
- Metal plasmon-coupled fluorescence imaging and label free coenzyme detection in cells.Biochemical and biophysical research communications · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The enzyme cofactors are intrinsically fluorescent and participate directly in the single molecule enzymology studies. Due to photobleaching, one cannot follow kinetics continuously by cofactor fluorescence for more than several minutes typically. Modification of spectral properties of fluorophores, such as the amplification of emission intensity, can be achieved through coupling with surface plasmons in close proximity to metallic nanostructures. This process, referred to as metal-enhanced fluorescence, offers promise for a range of applications, including bioassays, sensor technology, microarrays, and single-molecule studies. Here, we demonstrated up to a 100-fold increase in the emission of the single cofactors and flavoenzymes near silver nanostructures. Amplified fluorescence of different types of flavins and flavoenzymes has been interpreted by using time-resolved single molecule fluorescence data. The results show considerable promise for the studies of enzyme kinetics using the intrinsic fluorescence from the cofactors.
Identifiers
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Registered trials
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