ArticleMacromolecules2009
Trapping of Thiol Terminated Acrylate Polymers with Divinyl Sulfone to Generate Well-Defined Semi-Telechelic Michael Acceptor Polymers.
Article in Macromolecules, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 95 citations in OpenAlex.
- Synthesis of 5-Fluorouracil Polymer Conjugate andPolymers · 2023Article
- Tailored hyaluronic acid-based nanogels as theranostic boron delivery systems for boron neutron cancer therapy.International journal of pharmaceutics: X · 2022Article
- Poly(peptide): Synthesis, Structure, and Function of Peptide-Polymer Amphiphiles and Protein-like Polymers.Accounts of chemical research · 2020Article
- Polymer conjugation of proteins as a synthetic post-translational modification to impact their stability and activity.Polymer chemistry · 2019Article
- In Situ Quenching of Trialkylphosphine Reducing Agents Using Water-Soluble PEG-Azides Improves Maleimide Conjugation to Proteins.ACS omega · 2017Article
- Core/shell protein-reactive nanogels via a combination of RAFT polymerization and vinyl sulfone postmodification.Nanomedicine (London, England) · 2016Article
- Labeling and functionalizing amphipols for biological applications.The Journal of membrane biology · 2014Review
- Synthesis of photodegradable macromers for conjugation and release of bioactive molecules.Biomacromolecules · 2013Article
- Aminooxy and Pyridyl Disulfide Telechelic Poly(Polyethylene Glycol Acrylate) by RAFT Polymerization.Macromolecules · 2012Article
- Emerging synthetic approaches for protein-polymer conjugations.Chemical communications (Cambridge, England) · 2011Article
- A versatile method for functionalizing surfaces with bioactive glycans.Bioconjugate chemistry · 2011Article
- Protein-polymer conjugates: synthetic approaches by controlled radical polymerizations and interesting applications.Current opinion in chemical biology · 2010Review
- Heterotelechelic Polymers for Capture and Release of Protein-Polymer Conjugates.Journal of polymer science. Part A, Polymer chemistry · 2010Article
- Synthesis of Michael Acceptor Ionomers of Poly(4-Sulfonated Styrene-co-Poly(Ethylene Glycol) Methyl Ether Acrylate).Australian journal of chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Herein we report the synthesis of vinyl sulfone end functionalized PEGylated polymers by reversible addition-fragmentation chain transfer (RAFT) polymerization for conjugation to proteins. Poly(ethylene glycol) methyl ether acrylate (PEGA) was polymerized in the presence of 1-phenylethyl dithiobenzoate with 2,2'-azobis(2-methylpropionitrile) as the initiator to generate well-defined polyPEGAs with number-average molecular weights (M(n)) by gel permeation chromatography (GPC) of 6.7 kDa, 11.8 kDa and 16.1 kDa. Post-polymerization, the majority of polymer chains contained the dithioester functional group at the omega chain end, and the polydispersity indexes (PDI) of the polymers ranged from 1.08 to 1.24. The dithioester was subsequently reduced via aminolysis, and the resulting thiol was trapped with a divinyl sulfone in situ to produce semi-telechelic, vinyl sulfone polyPEGAs with efficiencies ranging between 85% and 99%. It was determined that the retention of vinyl sulfone was directly related to reaction time, with the maximum dithioester being transformed into a vinyl sulfone within 30 minutes. Longer reaction times resulted in slow decomposition of the vinyl sulfone end group. The resulting semi-telechelic vinyl sulfone polymers were then conjugated to a protein containing a free cysteine, bovine serum albumin (BSA). Gel electrophoresis demonstrated that the reaction was highly efficient and that conjugates of increasing size were readily prepared. After polymer attachment, the activity of the BSA was 92% of the unmodified biomolecule.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.