ArticlePlant & cell physiology2011
Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis.
Article in Plant & cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Whole-Genome Resequencing ofInternational journal of molecular sciences · 2026Article
- Mutation of OsKIF14.3, a Kinesin-14 Subfamily Protein, Altered Starch Metabolism and Caused Yellowish Leaf in Rice.International journal of molecular sciences · 2025Article
- Enrichment of phytoglycogen in sweet corn kernels through genomics-assisted selection.Functional & integrative genomics · 2025Article
- Filamentous Temperature-Sensitive Z Protein J175 Regulates Maize Chloroplasts' and Amyloplasts' Division and Development.Plants (Basel, Switzerland) · 2025Article
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Dynamics of starch formation and gene expression during grain filling and its possible influence on grain quality.Scientific reports · 2024Article
- Foliar Application of dsRNA Targeting Endogenous Potato (International journal of molecular sciences · 2022Article
- Dynamic Change in Starch Biosynthetic Enzymes Complexes during Grain-Filling Stages in BEIIb Active and Deficient Rice.International journal of molecular sciences · 2022Article
- Identifying loci controlling total starch content of leaf in Nicotiana tabacum through genome-wide association study.Functional & integrative genomics · 2022Article
- Post-genomics revolution in the design of premium quality rice in a high-yielding background to meet consumer demands in the 21st century.Plant communications · 2022Review
- Disruption ofInternational journal of molecular sciences · 2022Article
- Association among starch storage, metabolism, related genes and growth of Moso bamboo (Phyllostachys heterocycla) shoots.BMC plant biology · 2021Article
- A Systemic View of Carbohydrate Metabolism in Rice to Facilitate Productivity.Plants (Basel, Switzerland) · 2021Article
- A review of starch biosynthesis in cereal crops and its potential breeding applications in rice (PeerJ · 2021Article
- A Review of Starch Biosynthesis in Relation to the Building Block-Backbone Model.International journal of molecular sciences · 2020Review
- Functional Analysis of Starch Metabolism in Plants.Plants (Basel, Switzerland) · 2020Article
- Imaging Amyloplasts in the Developing Endosperm of Barley and Rice.Scientific reports · 2019Article
- Starch formation inside plastids of higher plants.Protoplasma · 2018Review
- Evolutionary, structural and expression analysis of core genes involved in starch synthesis.Scientific reports · 2018Article
- Proteome Analysis of Potato Starch Reveals the Presence of New Starch Metabolic Proteins as Well as Multiple Protease Inhibitors.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Debranching enzymes, which hydrolyze α-1 and 6-glucosidic linkages in α-polyglucans, play a dual role in the synthesis and degradation of starch in plants. A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night. An epitope-tagged ISA3 expressed in Escherichia coli exhibited hydrolytic activity on β-limit dextrin and amylopectin. We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm. ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm. Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant. Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules. Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic. These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice.
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