ArticleThe Journal of biological chemistry2011
Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Aerobicity stimulon iniScience · 2026Article
- Structure, Mutation, Functional Domain Roles and Medical Implications of Glycerol Kinase.The protein journal · 2026Review
- Enhanced nattokinase production byFrontiers in microbiology · 2025Article
- Evolution and stability of complex microbial communities driven by trade-offs.Molecular systems biology · 2024Review
- Plasticity of growth laws tunes resource allocation strategies in bacteria.PLoS computational biology · 2024Article
- Minireview: Engineering evolution to reconfigure phenotypic traits in microbes for biotechnological applications.Computational and structural biotechnology journal · 2023Review
- ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis.ACS synthetic biology · 2022Article
- Enhancing protein perdeuteration by experimental evolution of Escherichia coli K-12 for rapid growth in deuterium-based media.Protein science : a publication of the Protein Society · 2021Article
- Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi-carbon utilizing pathway in obligate methanotrophic bacteria.Microbial biotechnology · 2021Article
- Hierarchical and simultaneous utilization of carbon substrates: mechanistic insights, physiological roles, and ecological consequences.Current opinion in microbiology · 2021Review
- A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli.PLoS computational biology · 2021Article
- Experimental Evolution of Anticipatory Regulation inFrontiers in microbiology · 2021Article
- Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins.Biotechnology for biofuels · 2020Article
- Causal mutations from adaptive laboratory evolution are outlined by multiple scales of genome annotations and condition-specificity.BMC genomics · 2020Article
- Regulation underlying hierarchical and simultaneous utilization of carbon substrates by flux sensors in Escherichia coli.Nature microbiology · 2020Article
- Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species.Microbial biotechnology · 2020Review
- Proteomic Analyses Reveal New Insights on the Antimicrobial Mechanisms of Chitosan Biopolymers and Their Nanosized Particles againstInternational journal of molecular sciences · 2019Article
- DynamicME: dynamic simulation and refinement of integrated models of metabolism and protein expression.BMC systems biology · 2019Article
- Metabolome and molecular basis for carbohydrate increase and nitrate reduction in burley tobacco seedlings by glycerol through upregulating carbon and nitrogen metabolism.Scientific reports · 2018Article
- Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation.Biotechnology for biofuels · 2018Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Herein we measure the effect of four adaptive non-synonymous mutations to the glycerol kinase (glpK) gene on catalytic function and regulation, to identify changes that correlate to increased fitness in glycerol media. The mutations significantly reduce affinity for the allosteric inhibitor fructose-1,6-bisphosphate (FBP) and formation of the tetramer, which are structurally related, in a manner that correlates inversely with imparted fitness during growth on glycerol, which strongly suggests that these enzymatic parameters drive growth improvement. Counterintuitively, the glpK mutations also increase glycerol-induced auto-catabolite repression that reduces glpK transcription in a manner that correlates to fitness. This suggests that increased specific GlpK activity is attenuated by negative feedback on glpK expression via catabolite repression, possibly to prevent methylglyoxal toxicity. We additionally report that glpK mutations were fixed in 47 of 50 independent glycerol-adapted lineages. By far the most frequently mutated locus (nucleotide 218) was mutated in 20 lineages, strongly suggesting this position has an elevated mutation rate. This study demonstrates that fitness correlations can be used to interrogate adaptive processes at the protein level and to identify the regulatory constraints underlying selection and improved growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.