ArticleThe Journal of surgical research2012
Subnormothermic machine perfusion at both 20°C and 30°C recovers ischemic rat livers for successful transplantation.
Article in The Journal of surgical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Mitochondrial hyperoxidation contributes to warm ischemia-reperfusion injury in rat and pig livers.Communications medicine · 2026Article
- Small animalWorld journal of gastroenterology · 2026Review
- Equal performance of HTK-based and UW-based perfusion solutions in sub-normothermic liver machine perfusion.Scientific reports · 2025Article
- Beyond the icebox: modern strategies in organ preservation for transplantation.Clinical transplantation and research · 2024Review
- Beneficial Effects of Combined Use of Extracorporeal Membrane Oxygenation and Hypothermic Machine Perfusion in Porcine Donors after Cardiac Death for Liver Transplantation.Journal of clinical medicine · 2023Article
- Exceeding the Limits of Static Cold Storage in Limb Transplantation Using Subnormothermic Machine Perfusion.Journal of reconstructive microsurgery · 2023Article
- Current practice and novel approaches in organ preservation.Frontiers in transplantation · 2023Review
- Two Compartment Evaluation of Liver Grafts During Acellular Room Temperature Machine Perfusion (acRTMP) in a Rat Liver Transplant Model.Frontiers in medicine · 2022Article
- The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion.Scientific reports · 2021Article
- Advanced technologies for the preservation of mammalian biospecimens.Nature biomedical engineering · 2021Review
- Initial perfusate purification during subnormothermic machine perfusion for porcine liver donated after cardiac death.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2020Article
- Human Red Blood Cells as Oxygen Carriers to Improve Ex-Situ Liver Perfusion in a Rat Model.Journal of clinical medicine · 2019Article
- Cold flush after dynamic liver preservation protects against ischemic changes upon reperfusion - an experimental study.Transplant international : official journal of the European Society for Organ Transplantation · 2019Article
- Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity.BioMed research international · 2019Article
- Article
- Oxygen consumption during hypothermic and subnormothermic machine perfusions of porcine liver grafts after cardiac death.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2018Article
- A Comparative Study of Single and Dual Perfusion During End-ischemic Subnormothermic Liver Machine Preservation.Transplantation direct · 2018Article
- NormothermicJournal of clinical and translational hepatology · 2018Review
- A Small Animal Model of Ex Vivo Normothermic Liver Perfusion.Journal of visualized experiments : JoVE · 2018Article
- Organ preservation: from the past to the future.Acta pharmacologica Sinica · 2018Review
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Authors and funding
8 authors.
Funding
Abstract
backgroundUtilizing livers from donors after cardiac death could significantly expand the donor pool. We have previously shown that normothermic (37°C) extracorporeal liver perfusion significantly improves transplantation outcomes of ischemic rat livers. Here we investigate whether recovery of ischemic livers is possible using sub-normothermic machine perfusion at 20°C and 30°C.
methodsLivers from male Lewis rats were divided into five groups after 1 h of warm ischemia (WI): (1) WI only, (2) 5 h of static cold storage (SCS), or 5 h of MP at (3) 20°C, (4) 30°C, and (5) 37°C. Long-term graft performance was evaluated for 28 d post-transplantation. Acute graft performance was evaluated during a 2 h normothermic sanguineous reperfusion ex vivo. Fresh livers with 5 h of SCS were positive transplant controls while fresh livers were positive reperfusion controls.
resultsFollowing machine perfusion (MP) (Groups 3, 4, and 5), ischemically damaged livers could be orthotopically transplanted into syngeneic recipients with 100% survival (N ≥ 4) after 4 wk. On the other hand, animals from WI only, or WI + SCS groups all died within 24 h of transplantation. Fresh livers preserved using SCS had the highest alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the lowest bile production during reperfusion, while at 28 d post-transplantation, livers preserved at 20°C and 30°C had the highest total bilirubin values.
conclusionsMP at both 20°C and 30°C eliminated temperature control in perfusion systems and recovered ischemically damaged rat livers. Postoperatively, low transaminases suggest a beneficial effect of sub-normothermic perfusion, while rising total bilirubin levels suggest inadequate prevention of ischemia- or hypothermia-induced biliary damage.
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