ArticleFree radical biology & medicine2011
Reaction of hemoglobin with HOCl: mechanism of heme destruction and free iron release.
Article in Free radical biology & medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 85 citations in OpenAlex.
- Electrolyzed Water as a Biocide in Premise Water Networks: A Narrative Review and a Conceptual Framework.Pathogens (Basel, Switzerland) · 2026Review
- Heme-mediated selection of encapsulatedEmerging microbes & infections · 2025Article
- Evaluation of synergistic effect of Zerumbone with Cisplatin on anti-angiogenic potential in zebrafish model.Medical oncology (Northwood, London, England) · 2025Article
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- Oxidation of hemoproteins byMicrobiology spectrum · 2024Article
- The Key Role of GSH in Keeping the Redox Balance in Mammalian Cells: Mechanisms and Significance of GSH in Detoxification via Formation of Conjugates.Antioxidants (Basel, Switzerland) · 2023Review
- Mechanisms and physiological function of daily haemoglobin oxidation rhythms in red blood cells.The EMBO journal · 2023Article
- Oxidative Reactions Catalyzed by Hydrogen Peroxide Produced by Streptococcus pneumoniae and Other Streptococci Cause the Release and Degradation of Heme from Hemoglobin.Infection and immunity · 2022Article
- Potential Role of Zinc in the COVID-19 Disease Process and its Probable Impact on Reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Melatonin interferes with COVID-19 at several distinct ROS-related steps.Journal of inorganic biochemistry · 2021Review
- Effect of myoglobin, hemin, and ferric iron on quality of chicken breast meat.Animal bioscience · 2021Article
- A Multiple-Hit Hypothesis Involving Reactive Oxygen Species and Myeloperoxidase Explains Clinical Deterioration and Fatality in COVID-19.International journal of biological sciences · 2021Review
- Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid.Oxidative medicine and cellular longevity · 2019Article
- Melatonin prevents hypochlorous acid-mediated cyanocobalamin destruction and cyanogen chloride generation.Journal of pineal research · 2018Article
- Peptide Inhibitor of Complement C1 Inhibits the Peroxidase Activity of Hemoglobin and Myoglobin.International journal of peptides · 2017Article
- Diffused Intra-Oocyte Hydrogen Peroxide Activates Myeloperoxidase and Deteriorates Oocyte Quality.PloS one · 2015Article
- Linoleic acid induces red blood cells and hemoglobin damage via oxidative mechanism.International journal of clinical and experimental pathology · 2015Article
- Melatonin prevents myeloperoxidase heme destruction and the generation of free iron mediated by self-generated hypochlorous acid.PloS one · 2015Article
- Disruption of heme-peptide covalent cross-linking in mammalian peroxidases by hypochlorous acid.Journal of inorganic biochemistry · 2014Article
- On the source of the non-transferrin-bound iron which accumulates in packed red blood cell units during storage.Blood transfusion = Trasfusione del sangue · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Hypochlorous acid (HOCl) is generated by myeloperoxidase using chloride and hydrogen peroxide as substrates. HOCl and its conjugate base (OCl(-)) bind to the heme moiety of hemoglobin (Hb) and generate a transient ferric species whose formation and decay kinetics indicate it can participate in protein aggregation and heme destruction along with subsequent free iron release. The oxidation of the Hb heme moiety by OCl(-) was accompanied by marked heme destruction as judged by the decrease in and subsequent flattening of the Soret absorbance peak at 405 nm. HOCl-mediated Hb heme depletion was confirmed by HPLC analysis and in-gel heme staining. Exposure of Hb to increasing concentrations of HOCl produced a number of porphyrin degradation products resulting from oxidative cleavage of one or more of the carbon-methene bridges of the tetrapyrrole ring, as identified by their characteristic HPLC fluorescence and LC-MS. A nonreducing denaturing SDS-PAGE showed several degrees of protein aggregation. Similarly, porphyrin degradation products were identified after exposure of red blood cells to increasing concentrations of HOCl, indicating biological relevance of this finding. This work provides a direct link between Hb heme destruction and subsequent free iron accumulation, as occurs under inflammatory conditions where HOCl is formed in substantial amounts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.