Evidence map›Paper›PMID 2154968›Full record

ArticleThe Biochemical journal1990

Subcellular distribution of alpha 2-adrenergic receptors, pertussis-toxin substrate and adenylate cyclase in human platelets.

M A Zamorski, J C Ferraro, R R Neubig

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

M A ZamorskiDepartment of Pharmacology, University of Michigan School of Medicine, Ann Arbor 48109-0626.
J C Ferraro
R R Neubig
University of Michigan · US

Funding

DETERMINANTS OF A2 ADRENORECEPTOR-N-PROTEIN INTERACTIONSR01HL037551 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NEUBIG, RICHARD R · 1987 to 1989
–
NHLBI NIH HHS HL 37551NIDDK NIH HHS DK 022748
6 · The paper itself

Abstract

The subcellular distribution of the alpha 2-adrenergic receptor, pertussis-toxin substrates (Gi, the inhibitory G-protein) and adenylate cyclase was determined in human platelets. The alpha 2-adrenergic receptor and pertussis-toxin substrate activity codistribute with surface membranes identified by a novel fluorescent-lectin method. The platelet granule fractions did not contain detectable Gi. Only 2-4% of the total pertussis-toxin substrate activity appears in soluble fractions, and this amount was not increased upon addition of purified beta gamma units or after pretreatment of platelets with adrenaline. There is no evidence for compartmentation of the alpha 2-adrenergic receptor or Gi to account for the low-affinity component of agonist binding to the alpha 2-adrenergic receptor in human platelet membranes. Translocation of Gi from plasma membrane to platelet cytosol or granules does not appear to play any significant role in the mechanism of alpha 2-receptor-mediated platelet activation.

Indexed as

Adenylate Cyclase ToxinPertussis ToxinAdenylyl CyclasesBlood PlateletsCell MembraneElectrophoresis, Polyacrylamide GelEpinephrineFluorescent DyesHumansReceptors, Adrenergic, alphaSubstrate SpecificityVirulence Factors, BordetellaAdenylate Cyclase ToxinAdenylyl CyclasesEpinephrineFluorescent DyesPertussis ToxinReceptors, Adrenergic, alphaVirulence Factors, Bordetella

Identifiers

PMID2154968
PMCPMC1133698
OpenAlexW2340919599

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.