ArticleCurrent biology : CB2011
The requirement for the Dam1 complex is dependent upon the number of kinetochore proteins and microtubules.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Article
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- A phylogenetically-restricted essential cell cycle progression factor in the human pathogen Candida albicans.Nature communications · 2022Article
- Leveraging machine learning essentiality predictions and chemogenomic interactions to identify antifungal targets.Nature communications · 2021Article
- CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation.Genes · 2019Article
- Gene Essentiality Analyzed bymBio · 2018Article
- A Matter of Scale and Dimensions: Chromatin of Chromosome Landmarks in the Fungi.Microbiology spectrum · 2017Review
- A chromosome 4 trisomy contributes to increased fluconazole resistance in a clinical isolate of Candida albicans.Microbiology (Reading, England) · 2017Article
- Neocentromeres Provide Chromosome Segregation Accuracy and Centromere Clustering to Multiple Loci along a Candida albicans Chromosome.PLoS genetics · 2016Article
- Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis.PLoS genetics · 2016Article
- Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles.Molecular biology of the cell · 2015Article
- Centromeres Off the Hook: Massive Changes in Centromere Size and Structure Following Duplication of CenH3 Gene in Fabeae Species.Molecular biology and evolution · 2015Article
- Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.Chromosoma · 2015Article
- Silencing is noisy: population and cell level noise in telomere-adjacent genes is dependent on telomere position and sir2.PLoS genetics · 2014Article
- A tetraploid intermediate precedes aneuploid formation in yeasts exposed to fluconazole.PLoS biology · 2014Article
- A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo.PloS one · 2014Article
- Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences.Molecular biology of the cell · 2013Article
- Centromere-like regions in the budding yeast genome.PLoS genetics · 2013Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- Analysis of protein function in clinical C. albicans isolates.Yeast (Chichester, England) · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Dam1 complex attaches the kinetochore to spindle microtubules and is a processivity factor in vitro. In Saccharomyces cerevisiae, which has point centromeres that attach to a single microtubule, deletion of any Dam1 complex member results in chromosome segregation failures and cell death. In Schizosaccharomyces pombe, which has epigenetically defined regional centromeres that each attach to 3-5 kinetochore microtubules, Dam1 complex homologs are not essential. To determine why the complex is essential in some organisms and not in others, we used Candida albicans, a multimorphic yeast with regional centromeres that attach to a single microtubule. Interestingly, the Dam1 complex was essential in C. albicans, suggesting that the number of microtubules per centromere is critical for its requirement. Importantly, by increasing CENP-A expression levels, more kinetochore proteins and microtubules were recruited to the centromeres, which remained fully functional. Furthermore, Dam1 complex members became less crucial for growth in cells with extra kinetochore proteins and microtubules. Thus, the requirement for the Dam1 complex is not due to the DNA-specific nature of point centromeres. Rather, the Dam1 complex is less critical when chromosomes have multiple kinetochore complexes and microtubules per centromere, implying that it functions as a processivity factor in vivo as well as in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.