ArticleBioorganic & medicinal chemistry letters2011
Development of a fluorogenic sensor for activated Cdc42.
Article in Bioorganic & medicinal chemistry letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Monitoring GPCR conformation with GFP-inspired dyes.iScience · 2024Article
- Rapid detection ofScience translational medicine · 2018Article
- Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.Journal of molecular biology · 2016Article
- Ratiometric Imaging Using a Single Dye Enables Simultaneous Visualization of Rac1 and Cdc42 Activation.Journal of the American Chemical Society · 2016Article
- Studying Cellular Signal Transduction with OMIC Technologies.Journal of molecular biology · 2015Review
- Equilibrium and dynamic design principles for binding molecules engineered for reagentless biosensors.Analytical biochemistry · 2014Article
- Environment-sensing merocyanine dyes for live cell imaging applications.Bioconjugate chemistry · 2013Article
- FRET-capture: a sensitive method for the detection of dynamic protein interactions.Chembiochem : a European journal of chemical biology · 2013Article
- Chemical tools for studying directed cell migration.ACS chemical biology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Cdc42, a member of the Rho GTPase family, is a fundamental regulator of the actin cytoskeleton during cell migration. To generate a sensor for Cdc42 activation, we employed a multi-pronged approach, utilizing cysteine labeling and expressed protein ligation, to incorporate the environment sensitive fluorophore 4-N,N-dimethylamino-1,8-naphthalimide (4-DMN) into the GTPase binding domain of the WASP protein. These constructs bind only the active, GTP-bound conformation of Cdc42 to produce a fluorescence signal. Studies with a panel of five sensor analogs revealed a derivative that exhibits a 32-fold increase in fluorescence intensity in the presence of activated Cdc42 compared to incubation with the inactive GDP-bound form of the protein. We demonstrate that this sensor can be exploited to monitor Cdc42 nucleotide exchange and GTPase activity in a continuous, fluorescence assay.
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Registered trials
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