ArticleBiomaterials2011
Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
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- Time-Dependent Material Properties and Composition of the Nonhuman Primate Uterine Layers Through Gestation.bioRxiv : the preprint server for biology · 2025Article
- Quality Dynamics of Beef Bottom Round During 2-Month Frozen Storage (-18 °C) and Week-Long Refrigeration (4 °C).Metabolites · 2025Article
- A method for analyzing AFM force mapping data obtained from soft tissue cryosections.Journal of biomechanics · 2024Article
- Experimental and computational analysis of the injection-induced mechanical changes in the skin microenvironment during subcutaneous injection of biologics.Extreme Mechanics Letters · 2023Article
- Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants.ACS applied materials & interfaces · 2023Article
- Decellularized xenogenic bone graft for repair of segmental bone defect in rabbits.Iranian journal of veterinary research · 2022Article
- Application of decellularized vascular matrix in small-diameter vascular grafts.Frontiers in bioengineering and biotechnology · 2022Review
- Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts.Materials (Basel, Switzerland) · 2020Article
- Role of intracellular poroelasticity on freezing-induced deformation of cells in engineered tissues.Journal of the Royal Society, Interface · 2016Article
- Characterization of Cell-Type-Specific Drug Transport and Resistance of Breast Cancers Using Tumor-Microenvironment-on-Chip.Molecular pharmaceutics · 2016Article
- Preservation of tissue microstructure and functionality during freezing by modulation of cytoskeletal structure.Journal of the mechanical behavior of biomedical materials · 2015Article
- The microenvironment of embryoid bodies modulated the commitment to neural lineage postcryopreservation.Tissue engineering. Part C, Methods · 2015Article
- Simulation of complex transport of nanoparticles around a tumor using tumor-microenvironment-on-chip.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Measurement of spatiotemporal intracellular deformation of cells adhered to collagen matrix during freezing of biomaterials.Journal of biomechanical engineering · 2014Article
- Role of cells in freezing-induced cell-fluid-matrix interactions within engineered tissues.Journal of biomechanical engineering · 2013Article
- Cryopreservation effects on recombinant myoblasts encapsulated in adhesive alginate hydrogels.Acta biomaterialia · 2013Article
- Spatiotemporal Characterization of Extracellular Matrix Microstructures in Engineered Tissue: A Whole-Field Spectroscopic Imaging Approach.Journal of nanotechnology in engineering and medicine · 2013Article
- Thermomechanical analysis of freezing-induced cell-fluid-matrix interactions in engineered tissues.Journal of the mechanical behavior of biomedical materials · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In this study, freezing-induced cell-fluid-matrix interactions and their impact on the cells and ECM microstructure of ETs were investigated using dermal equivalents as a model ET. The dermal equivalents were constructed by seeding human dermal fibroblasts in type I collagen matrices with varying cell seeding density and collagen concentration. While these dermal equivalents underwent an identical freeze/thaw condition, their spatiotemporal deformation during freezing, post-thaw ECM microstructure, and cellular level cryoresponse were characterized. The results showed that the extent and characteristics of freezing-induced deformation were significantly different among the experimental groups, and the ETs with denser ECM microstructure experienced a larger deformation. The magnitude of the deformation was well correlated to the post-thaw ECM structure, suggesting that the freezing-induced deformation is a good indicator of post-thaw ECM structure. A significant difference in the extent of cellular injury was also noted among the experimental groups, and it depended on the extent of freezing-induced deformation of the ETs and the initial cytoskeleton organization. These results suggest that the cells have been subjected to mechanical insult due to the freezing-induced deformation as well as thermal insult. These findings provide insight on tissue-type dependent cryopreservation outcomes, and can help to design and modify cryopreservation protocols for new types of tissues from a pre-developed cryopreservation protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.