ArticleVirology journal2011
Enhancement of bacteriolysis of shuffled phage PhiX174 gene E.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Bacterial Systems as a Precision Delivery Platform of Therapeutic Peptides for Cancer Therapy.Polymer science & technology (Washington, D.C.) · 2025Review
- Comparative analysis of Escherichia coli Nissle 1917 ghosts quality: a study of two chemical methods.Archives of microbiology · 2024Article
- Bacteriophage-encoded protein utilization in bacterial ghost production: a mini-review.World journal of microbiology & biotechnology · 2024Review
- Comparison of the immune effects of theFrontiers in microbiology · 2024Article
- Preparation of Escherichia coli ghost of anchoring bovine Pasteurella multocida OmpH and its immunoprotective effect.BMC veterinary research · 2023Article
- Efficient Robust Yield Method for Preparing Bacterial Ghosts byBioengineering (Basel, Switzerland) · 2022Article
- Bacterial Ghosts-Based Vaccine and Drug Delivery Systems.Pharmaceutics · 2021Review
- Article
- A novel autolysis system for extracellular production and direct immobilization of a phospholipase D fused with cellulose binding domain.BMC biotechnology · 2019Article
- Bacteriophages and phage-derived proteins--application approaches.Current medicinal chemistry · 2015Review
- Article
- Minimal requirements for inhibition of MraY by lysis protein E from bacteriophage ΦX174.Molecular microbiology · 2012Article
- Generation of biotechnology-derived Flavobacterium columnare ghosts by PhiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp.Journal of biomedicine & biotechnology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial ghosts that are generated using the regulated PhiX174 lysis gene E offer a new avenue for the study of inactivated vaccines. Here, we constructed a library of mutant gene E using a gene-shuffling technique. After screening and recombination with the prokaryotic non-fusion expression vector pBV220, two lysis plasmids were selected. Among which, a novel mutant E gene (named mE), consisting of a 74-bp non-encoding sequence at 5'-end and a 201-bp gene ΔE, significantly increased the lysis effect on prokaryotic Escherichia coli and Salmonella enteritidis. Moreover, lysis efficiency, as measured by the OD600 value, reached 1.0 (10⁹ CFU), avoiding the bottleneck problem observed with other bacterial lysis procedures, which results in a low concentration of bacteria in suspension, and consequent low production of bacterial ghosts. Our results may provide a promising avenue for the development of bacterial ghost vaccines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.