ArticleBMC research notes2011
Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Efficient genome editing in a Mozambique tilapia cell line using CAS ribonucleoprotein complexes.Scientific reports · 2026Article
- Orai1 and Orai3 act through distinct signalling axes to promote stemness and tumorigenicity of breast cancer stem cells.Stem cell research & therapy · 2024Article
- Transfection, cytotoxicity, and cell cycle studies on the two newly developed and characterized humpback grouper (Cromileptes altivelis) fin cell lines.In vitro cellular & developmental biology. Animal · 2024Article
- Efficient transfection of Atlantic salmon primary hepatocyte cells for functional assays and gene editing.G3 (Bethesda, Md.) · 2023Article
- Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878).Fish physiology and biochemistry · 2022Article
- Role and relevance of fish cell lines in advanced in vitro research.Molecular biology reports · 2022Review
- Generation of Self-Inhibitory Recombinant Viral Hemorrhagic Septicemia Virus (VHSV) by Insertion of Viral P Gene-Targeting Artificial MicroRNA into Viral Genome and Effect of Dicer Gene Knockout on the Recombinant VHSV Replication.Marine biotechnology (New York, N.Y.) · 2021Article
- Efficient Genome Editing in Multiple Salmonid Cell Lines Using Ribonucleoprotein Complexes.Marine biotechnology (New York, N.Y.) · 2020Article
- Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.).Fish physiology and biochemistry · 2019Article
- New reverse genetics and transfection methods to rescue arboviruses in mosquito cells.Scientific reports · 2017Article
- Functional xenobiotic metabolism and efflux transporters in trout hepatocyte spheroid cultures.Toxicology research · 2015Article
- A method to evaluate the efficiency of transfection reagents in an adherent zebrafish cell line.BioResearch open access · 2013Article
- Gravity-oriented microfluidic device for uniform and massive cell spheroid formation.Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium phosphate precipitation or lipid-based reagents shows very low transfection efficiency. The Amaxa Nucleofector technology™ is an electroporation technique that has been shown to be efficient for gene transfer into primary cells and hard to transfect cell lines.
findingsHere we demonstrate, enhanced transfection of the head kidney cell line, TO, from Atlantic salmon using nucleofection and subsequent flow cytometry. Depending on the plasmid promoter, TO cells could be transfected transiently with an efficiency ranging from 11.6% to 90.8% with good viability, using Amaxa's cell line nucleofector solution T and program T-20. A kill curve was performed to investigate the most potent antibiotic for selection of transformed cells, and we found that blasticidin and puromycin were the most efficient for selection of TO cells.
conclusionsThe results show that nucleofection is an efficient way of gene transfer into Atlantic salmon cells and that stably transfected cells can be selected with blasticidin or puromycin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.