ArticleInternational journal of biological sciences2011
Identification of pluripotency genes in the fish medaka.
Article in International journal of biological sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 76 citations in OpenAlex.
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- Emerging Frontiers in Zebrafish Embryonic and Adult-Derived Cell Lines.International journal of molecular sciences · 2025Review
- Pluripotent cells derived from 50% epiboly zebrafish embryos.In vitro cellular & developmental biology. Animal · 2025Article
- Research progress of nanog gene in fish.Molecular genetics and genomics : MGG · 2024Review
- Establishment and Characterization of a Spermatogonial Stem Cell Line from Tiger Puffer Fish (Animals : an open access journal from MDPI · 2023Article
- Notch pathway is required for protection against heat stress in spermatogonial stem cells in medaka.Fish physiology and biochemistry · 2023Article
- The Divergent and Conserved Expression Profile of TurtleBiology · 2022Article
- Characterization of the Nanog gene involved in the gonadal development in pearlscale angelfish (Centropyge vrolikii).Fish physiology and biochemistry · 2022Article
- Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming.Frontiers in genetics · 2022Article
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- The transcription factor Zfp281 sustains CD4Cellular & molecular immunology · 2020Article
- Enhanced Enrichment of Medaka Ovarian Germline Stem Cells by a Combination of Density Gradient Centrifugation and Differential Plating.Biomolecules · 2020Article
- MeagreAnimals : an open access journal from MDPI · 2020Article
- Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems.Biomolecules · 2020Review
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- Screening for Antiviral Medaka Haploid Embryonic Stem Cells by Genome Wide Mutagenesis.Marine biotechnology (New York, N.Y.) · 2019Article
- The function of tcf3 in medaka embryos: efficient knockdown with pePNAs.BMC biotechnology · 2018Article
- Evolutionary Conservation of pou5f3 Genomic Organization and Its Dynamic Distribution during Embryogenesis and in Adult Gonads in Japanese Flounder Paralichthys olivaceus.International journal of molecular sciences · 2017Article
- Transcriptome analysis reveals differentially expressed genes associated with germ cell and gonad development in the Southern bluefin tuna (Thunnus maccoyii).BMC genomics · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cell cultures can be derived directly from early developing embryos and indirectly from differentiated cells by forced expression of pluripotency transcription factors. Pluripotency genes are routinely used to characterize mammalian stem cell cultures at the molecular level. However, such genes have remained unknown in lower vertebrates. In this regard, the laboratory fish medaka is uniquely suited because it has embryonic stem (ES) cells and genome sequence data. We identified seven medaka pluripotency genes by homology search and expression in vivo and in vitro. By RT-PCR analysis, the seven genes fall into three groups of expression pattern. Group I includes nanog and oct4 showing gonad-specific expression; Group II contains sall4 and zfp281 displaying gonad-preferential expression; Group III has klf4, ronin and tcf3 exhibiting expression also in several somatic tissues apart from the gonads. The transcripts of the seven genes are maternally supplied and persist at a high level during early embryogenesis. We made use of early embryos and adult gonads to examine expression in stem cells and differentiated derivatives by in situ hybridization. Strikingly, nanog and oct4 are highly expressed in pluripotent blastomeres of 16-cell embryos. In the adult testis, nanog expression was specific to spermatogonia, the germ stem cells, whereas tcf3 expression occurred in spermatogonia and differentiated cells. Most importantly, all the seven genes are pluripotency markers in vitro, because they have high expression in undifferentiated ES cells but dramatic down-regulation upon differentiation. Therefore, these genes have conserved their pluripotency-specific expression in vitro from mammals to lower vertebrates.
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