ArticleInternational journal of biological sciences2011
Medaka cleavage embryos are capable of generating ES-like cell cultures.
Article in International journal of biological sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming.Frontiers in genetics · 2022Article
- Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro.Scientific reports · 2017Article
- Dnd Is a Critical Specifier of Primordial Germ Cells in the Medaka Fish.Stem cell reports · 2016Article
- Article
- Massive production of all-female diploids and triploids in the crucian carp.International journal of biological sciences · 2011Article
- Fishing fish stem cells and nuclear transplants.International journal of biological sciences · 2011Article
- Identification of pluripotency genes in the fish medaka.International journal of biological sciences · 2011Article
- Fish stem cell cultures.International journal of biological sciences · 2011Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
Mammalian embryos at the blastocyst stage have three major lineages, which in culture can give rise to embryonic stem (ES) cells from the inner cell mass or epiblast, trophoblast stem cells from the trophectoderm, and primitive endoderm stem cells. None of these stem cells is totipotent, because they show gene expression profiles characteristic of their sources and usually contribute only to the lineages of their origins in chimeric embryos. It is unknown whether embryos prior to the blastocyst stage can be cultivated towards totipotent stem cell cultures. Medaka is an excellent model for stem cell research. This laboratory fish has generated diploid and even haploid ES cells from the midblastula embryo with ~2000 cells. Here we report in medaka that dispersed cells from earlier embryos can survive, proliferate and attach in culture. We show that even 32-cells embryos can be dissociated into individual cells capable of producing continuously growing ES-like cultures. Our data point to the possibility to derive stable cell culture from cleavage embryos in this organism.
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