ArticleMolecular oral microbiology2011
Interaction of oral bacteria with gingival epithelial cell multilayers.
Article in Molecular oral microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- Relationship between invasion of the periodontium by periodontal pathogens and periodontal disease: a systematic review.Virulence · 2015Pooled it
- Impact of E-Liquids on Phagocytosis, Escape and Invasion ofPathogens (Basel, Switzerland) · 2026Article
- Porphyromonas gingivalis Suppresses Interferon-Gamma Signaling in Macrophages Through a Contact-Dependent, Gingipain-Mediated Mechanism.MicrobiologyOpen · 2026Article
- Review
- Mechanical-enzymatic isolation and characterization of primary human gingival epithelial cells for reproducible in vitro oral mucosa models.Histochemistry and cell biology · 2025Article
- An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.Microbiome · 2025Article
- Underscoring long-term host-microbiome interactions in a physiologically relevant gingival tissue model.NPJ biofilms and microbiomes · 2025Article
- Periodontal pathogens and cancer development.Periodontology 2000 · 2024Review
- Role of ferroptosis inJournal of oral microbiology · 2024Article
- An update on periodontal inflammation and bone loss.Frontiers in immunology · 2024Review
- Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis.Journal of oral microbiology · 2024Article
- Experimental Modeling of Host-Bacterial Interactions in Head and Neck Squamous Cell Carcinoma.Cancers · 2023Review
- New Trends in the Impact of Periodontal Treatment on Early Cardiovascular Diseases Outcomes: Insights and Future Perspectives.Reviews in cardiovascular medicine · 2023Review
- A physiologically relevant culture platform for long-term studies of in vitro gingival tissue.Acta biomaterialia · 2023Article
- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Three-Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- IL-8 Secreted by Gastric Epithelial Cells Infected withViruses · 2023Article
- Stable reconstructed human gingiva-microbe interaction model: Differential response to commensals and pathogens.Frontiers in cellular and infection microbiology · 2022Article
- Human Oral Keratinocytes Challenged byInternational journal of microbiology · 2022Article
- Pathogenic Mechanisms ofFrontiers in oral health · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. Porphyromonas gingivalis invaded intracellularly and spread from cell to cell; A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement through the multilayer; whereas S. gordonii remained extracellular and predominantly associated with the superficial cell layer. None of the bacterial species disrupted barrier function as measured by transepithelial electrical resistance. P. gingivalis did not elicit secretion of proinflammatory cytokines. However, A. actinomycetemcomitans and S. gordonii induced interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 secretion; and F. nucleatum stimulated production of IL-1β and TNF-α. Aggregatibacter actinomycetemcomitans, F. nucleatum and S. gordonii, but not P. gingivalis, increased levels of apoptosis after 24 h infection. The results indicate that the organisms with pathogenic potential were able to traverse the epithelium, whereas the commensal bacteria did not. In addition, distinct host responses characterized the interaction between the junctional epithelium and oral bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.