ArticleExperimental dermatology2011
Spatial mapping by imaging mass spectrometry offers advancements for rapid definition of human skin proteomic signatures.
Article in Experimental dermatology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Desorption electrospray ionization and matrix-assisted laser desorption/ionization as imaging approaches for biological samples analysis.Analytical and bioanalytical chemistry · 2023Review
- Diagnosis of melanoma by imaging mass spectrometry: Development and validation of a melanoma prediction model.Journal of cutaneous pathology · 2021Article
- Screening of differentially expressed proteins in psoriasis vulgaris by two-dimensional gel electrophoresis and mass spectrometry.Experimental and therapeutic medicine · 2017Article
- The Need for Speed in Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Postdoc journal : a journal of postdoctoral research and postdoctoral affairs · 2016Article
- Histology-guided protein digestion/extraction from formalin-fixed and paraffin-embedded pressure ulcer biopsies.Experimental dermatology · 2016Article
- Histology-directed and imaging mass spectrometry: An emerging technology in ectopic calcification.Bone · 2015Article
- Imaging mass spectrometry for assessing cutaneous wound healing: analysis of pressure ulcers.Journal of proteome research · 2015Article
- Histology-directed microwave assisted enzymatic protein digestion for MALDI MS analysis of mammalian tissue.Analytical chemistry · 2015Article
- Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research.Chemical reviews · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Investigations into the human skin proteome by classical analytical procedures have not addressed spatial molecular distributions in whole-skin biopsies. The aim of this study was to develop methods for the detection of protein signatures and their spatial disposition in human skin using advanced molecular imaging technology based on mass spectrometry technologies. This technology allows for the generation of protein images at specific molecular weight values without the use of antibody while maintaining tissue architecture. Two experimental approaches were employed: MALDI-MS profiling, where mass spectra were taken from discrete locations based on histology, and MALDI-IMS imaging, where complete molecular images were obtained at various MW values. In addition, proteins were identified by in situ tryptic digestion, sequence analysis of the fragment peptides and protein database searching. We have detected patterns of protein differences that exist between epidermis and dermis as well as subtle regional differences between the papillary and reticular dermis. Furthermore, we were able to detect proteins that are constitutive features of human skin as well as those associated with unique markers of individual variability.
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