ArticleAnalytical chemistry2011
Rapid detection of urinary tract infections using isotachophoresis and molecular beacons.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 95 citations in OpenAlex.
- Microvalve-Based Tunability of Electrically Driven Ion Transport through a Microfluidic System with an Ion-Exchange Membrane.Analytical chemistry · 2023Article
- Isotachophoresis: Theory and Microfluidic Applications.Chemical reviews · 2022Review
- Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2021Article
- Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- One-Step Nucleic Acid Purification and Noise-Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow-Abundance Nucleic Acid Detection.Angewandte Chemie (International ed. in English) · 2020Article
- Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer.Lab on a chip · 2019Article
- Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens.The Analyst · 2019Article
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Depletion of cells and abundant proteins from biological samples by enhanced dielectrophoresis.Sensors and actuators. B, Chemical · 2014Article
- Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip.Analytical chemistry · 2013Article
- Advances in microfluidic materials, functions, integration, and applications.Chemical reviews · 2013Review
- Rapid hybridization of nucleic acids using isotachophoresis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- On-chip isotachophoresis for separation of ions and purification of nucleic acids.Journal of visualized experiments : JoVE · 2012Article
- Micro total analysis systems for cell biology and biochemical assays.Analytical chemistry · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria specific sequences. We demonstrated detection of E. coli in bacteria cultures as well as in patient urine samples in the clinically relevant range 1E6-1E8 cfu/mL. For bacterial cultures we further demonstrate quantification in this range. The assay requires minimal sample preparation (a single centrifugation and dilution), and can be completed, from beginning of lysing to detection, in under 15 min. We believe that the principles presented here can be used for design of other rapid diagnostics or detection methods for pathogenic diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.