ArticlePloS one2011
Development of a high-throughput Candida albicans biofilm chip.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- In Situ Aqueous Spice Extract-Based Antifungal Lock Strategy for Salvage of Foley's Catheter Biofouled withGels (Basel, Switzerland) · 2025Article
- Host-microbe-cancer interactions on-a-chip.Frontiers in bioengineering and biotechnology · 2025Review
- Virulence and biofilms as promising targets in developing antipathogenic drugs against candidiasis.Future science OA · 2020Review
- Candida albicans biofilm growth and dispersal: contributions to pathogenesis.Current opinion in microbiology · 2019Review
- Nano-biofilm Arrays as a Novel Universal Platform for Microscale Microbial Culture and High-Throughput Downstream Applications.Current medicinal chemistry · 2019Review
- Methodologies forMicrobial cell (Graz, Austria) · 2018Review
- Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions.Lab on a chip · 2018Article
- Article
- Plasticity of Candida albicans Biofilms.Microbiology and molecular biology reviews : MMBR · 2016Review
- Candida Biofilms: Development, Architecture, and Resistance.Microbiology spectrum · 2015Review
- From Biology to Drug Development: New Approaches to Combat the Threat of Fungal Biofilms.Microbiology spectrum · 2015Article
- Sustained release of a novel anti-quorum-sensing agent against oral fungal biofilms.Antimicrobial agents and chemotherapy · 2015Article
- Synergistic interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an in vitro wound model.Infection and immunity · 2014Article
- Overcoming antifungal resistance.Drug discovery today. Technologies · 2014Review
- Drug susceptibility of matrix-encapsulated Candida albicans nano-biofilms.Biotechnology and bioengineering · 2014Article
- Therapeutic potential of thiazolidinedione-8 as an antibiofilm agent against Candida albicans.PloS one · 2014Article
- Article
- Candidiasis drug discovery and development: new approaches targeting virulence for discovering and identifying new drugs.Expert opinion on drug discovery · 2013Review
- Article
- Candida albicans biofilm chip (CaBChip) for high-throughput antifungal drug screening.Journal of visualized experiments : JoVE · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a high-density microarray platform consisting of nano-biofilms of Candida albicans. A robotic microarrayer was used to print yeast cells of C. albicans encapsulated in a collagen matrix at a volume as low as 50 nL onto surface-modified microscope slides. Upon incubation, the cells grow into fully formed "nano-biofilms". The morphological and architectural complexity of these biofilms were evaluated by scanning electron and confocal scanning laser microscopy. The extent of biofilm formation was determined using a microarray scanner from changes in fluorescence intensities due to FUN 1 metabolic processing. This staining technique was also adapted for antifungal susceptibility testing, which demonstrated that, similar to regular biofilms, cells within the on-chip biofilms displayed elevated levels of resistance against antifungal agents (fluconazole and amphotericin B). Thus, results from structural analyses and antifungal susceptibility testing indicated that despite miniaturization, these biofilms display the typical phenotypic properties associated with the biofilm mode of growth. In its final format, the C. albicans biofilm chip (CaBChip) is composed of 768 equivalent and spatially distinct nano-biofilms on a single slide; multiple chips can be printed and processed simultaneously. Compared to current methods for the formation of microbial biofilms, namely the 96-well microtiter plate model, this fungal biofilm chip has advantages in terms of miniaturization and automation, which combine to cut reagent use and analysis time, minimize labor intensive steps, and dramatically reduce assay costs. Such a chip should accelerate the antifungal drug discovery process by enabling rapid, convenient and inexpensive screening of hundreds-to-thousands of compounds simultaneously.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.