ArticleActa crystallographica. Section D, Biological crystallography2011
Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening.
Article in Acta crystallographica. Section D, Biological crystallography, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Molecular basis for dual functions in pilus assembly modulated by the lid of a pilus-specific sortase.The Journal of biological chemistry · 2024Article
- Article
- A cryptic oxidoreductase safeguards oxidative protein folding inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The FUSION protein crystallization screen.Journal of applied crystallography · 2022Article
- Structural Basis of a Thiol-Disulfide Oxidoreductase in the Hedgehog-Forming Actinobacterium Corynebacterium matruchotii.Journal of bacteriology · 2018Article
- Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology.Journal of visualized experiments : JoVE · 2018Article
- Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.Autophagy · 2016Article
- Protein crystallization screens developed at the MRC Laboratory of Molecular Biology.Drug discovery today · 2016Review
- A thiol-disulfide oxidoreductase of the Gram-positive pathogen Corynebacterium diphtheriae is essential for viability, pilus assembly, toxin production and virulence.Molecular microbiology · 2015Article
- A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris.The Journal of biological chemistry · 2015Article
- Sparse and incomplete factorial matrices to screen membrane protein 2D crystallization.Journal of structural biology · 2015Article
- Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity.Molecular microbiology · 2015Article
- Article
- Porous nucleating agents for protein crystallization.Nature protocols · 2014Article
- The current approach to initial crystallization screening of proteins is under-sampled.Journal of applied crystallography · 2013Article
- Agonist-bound adenosine A2A receptor structures reveal common features of GPCR activation.Nature · 2011Article
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4 authors.
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Abstract
The Pi sampling method is derived from the incomplete factorial approach to macromolecular crystallization screen design. The resulting `Pi screens' have a modular distribution of a given set of up to 36 stock solutions. Maximally diverse conditions can be produced by taking into account the properties of the chemicals used in the formulation and the concentrations of the corresponding solutions. The Pi sampling method has been implemented in a web-based application that generates screen formulations and recipes. It is particularly adapted to screens consisting of 96 different conditions. The flexibility and efficiency of Pi sampling is demonstrated by the crystallization of soluble proteins and of an integral membrane-protein sample.
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