ArticlePlant physiology2011
Application of gene targeting to designed mutation breeding of high-tryptophan rice.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 66 citations in OpenAlex.
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Beyond the genome: the role of functional markers in contemporary plant breeding.Frontiers in plant science · 2025Review
- Genome Editing Technology and Its Application to Metabolic Engineering in Rice.Rice (New York, N.Y.) · 2022Review
- Plant Genome Editing and the Relevance of Off-Target Changes.Plant physiology · 2020Article
- Genetic Manipulation for Improved Nutritional Quality in Rice.Frontiers in genetics · 2020Review
- Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research.Biodesign research · 2020Review
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- Improved growth performance, food efficiency, and lysine availability in growing rats fed with lysine-biofortified rice.Scientific reports · 2017Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Improving Nutritional Quality of Plant Proteins Through Genetic Engineering.Current genomics · 2016Article
- Integrated metabolomics and phytochemical genomics approaches for studies on rice.GigaScience · 2016Review
- Whole-genome analysis of herbicide-tolerant mutant rice generated by Agrobacterium-mediated gene targeting.Plant & cell physiology · 2015Article
- Precision genome editing in plants via gene targeting and piggyBac-mediated marker excision.The Plant journal : for cell and molecular biology · 2015Article
- Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat.Protoplasma · 2014Article
- Precise marker excision system using an animal-derived piggyBac transposon in plants.The Plant journal : for cell and molecular biology · 2014Article
- The non-homologous end-joining pathway is involved in stable transformation in rice.Frontiers in plant science · 2014Review
- Changes in primary and secondary metabolite levels in response to gene targeting-mediated site-directed mutagenesis of the anthranilate synthase gene in rice.Metabolites · 2012Article
- Insights into the regenerative property of plant cells and their receptivity to transgenesis: wheat as a research case study.Plant signaling & behavior · 2012Review
- In planta gene targeting.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multiple roles of the ER stress sensor IRE1 demonstrated by gene targeting in rice.Scientific reports · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-directed mutagenesis via gene targeting (GT) based on homologous recombination is the ultimate mutation breeding technology because it enables useful information acquired from structural- and computational-based protein engineering to be applied directly to molecular breeding, including metabolic engineering, of crops. Here, we employed this rationale to introduce precise mutations in OASA2--an α-subunit of anthranilate synthase that is a key enzyme of tryptophan (Trp) biosynthesis in rice (Oryza sativa)--via GT, with subsequent selection of GT cells using a Trp analog. The expression level of OASA2 in plants homozygous and heterozygous for modified OASA2 was similar to that of nontransformants, suggesting that OASA2 transcription in GT plants was controlled in the same manner as endogenous OASA2, and that GT could lead to a lower risk of gene silencing than in conventional overexpression approaches. Moreover, we showed that enzymatic properties deduced from protein engineering or in vitro analysis could be reproduced in GT plants as evidenced by Trp accumulation levels. Interestingly, mature seeds of homozygous GT plants accumulated Trp levels 230-fold higher than in nontransformants without any apparent morphological or developmental changes. Thus, we have succeeded in producing a novel rice plant of great potential nutritional benefit for both man and livestock that could not have been selected using conventional mutagenesis approaches. Our results demonstrate the effectiveness of directed crop improvement by combining precision mutagenesis via GT with a knowledge of protein engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.