ArticleJournal of experimental botany2011
Acyl substrate preferences of an IAA-amido synthetase account for variations in grape (Vitis vinifera L.) berry ripening caused by different auxinic compounds indicating the importance of auxin conjugation in plant development.
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 103 citations in OpenAlex.
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- Genome-wide comprehensive analysis the molecular phylogenetic evolution, functional divergence and tissue-specific expression ofFrontiers in plant science · 2025Article
- VvFHY3 links auxin and endoplasmic reticulum stress to regulate grape anthocyanin biosynthesis at high temperatures.The Plant cell · 2024Article
- Genome-wide identification and expression characterization of the GH3 gene family of tea plant (Camellia sinensis).BMC genomics · 2024Article
- Non-climacteric fruit development and ripening regulation: 'the phytohormones show'.Journal of experimental botany · 2023Review
- Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue.Plant physiology · 2023Article
- Integrative analysis of grapevine (Frontiers in nutrition · 2023Article
- Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit.The New phytologist · 2022Article
- VviPLATZ1 is a major factor that controls female flower morphology determination in grapevine.Nature communications · 2021Article
- Genome-Wide Analysis, Modeling, and Identification of Amino Acid Binding Motifs Suggest the Involvement ofPlants (Basel, Switzerland) · 2021Article
- The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development inInternational journal of molecular sciences · 2021Article
- The role of auxin during early berry development in grapevine as revealed by transcript profiling from pollination to fruit set.Horticulture research · 2021Article
- Functional conservation of the grapevine candidate gene INNER NO OUTER for ovule development and seed formation.Horticulture research · 2021Article
- Exogenous 2,4-Epibrassinolide Treatment Maintains the Quality of Carambola Fruit Associated With Enhanced Antioxidant Capacity and Alternative Respiratory Metabolism.Frontiers in plant science · 2021Article
- Gibberellic Acid Modifies the Transcript Abundance of ABA Pathway Orthologs and Modulates Sweet Cherry (Plants (Basel, Switzerland) · 2020Article
- Article
- Auxin treatment of grapevine (Vitis vinifera L.) berries delays ripening onset by inhibiting cell expansion.Plant molecular biology · 2020Article
- Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2019Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nine Gretchen Hagen (GH3) genes were identified in grapevine (Vitis vinifera L.) and six of these were predicted on the basis of protein sequence similarity to act as indole-3-acetic acid (IAA)-amido synthetases. The activity of these enzymes is thought to be important in controlling free IAA levels and one auxin-inducible grapevine GH3 protein, GH3-1, has previously been implicated in the berry ripening process. Ex planta assays showed that the expression of only one other GH3 gene, GH3-2, increased following the treatment of grape berries with auxinic compounds. One of these was the naturally occurring IAA and the other two were synthetic, α-naphthalene acetic acid (NAA) and benzothiazole-2-oxyacetic acid (BTOA). The determination of steady-state kinetic parameters for the recombinant GH3-1 and GH3-2 proteins revealed that both enzymes efficiently conjugated aspartic acid (Asp) to IAA and less well to NAA, while BTOA was a poor substrate. GH3-2 gene expression was induced by IAA treatment of pre-ripening berries with an associated increase in levels of IAA-Asp and a decrease in free IAA levels. This indicates that GH3-2 responded to excess auxin to maintain low levels of free IAA. Grape berry ripening was not affected by IAA application prior to veraison (ripening onset) but was considerably delayed by NAA and even more so by BTOA. The differential effects of the three auxinic compounds on berry ripening can therefore be explained by the induction and acyl substrate specificity of GH3-2. These results further indicate an important role for GH3 proteins in controlling auxin-related plant developmental processes.
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