ArticleJournal of virology2011
The domain I-domain III linker plays an important role in the fusogenic conformational change of the alphavirus membrane fusion protein.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Fusion Inhibition of Zika Virus Entry by a Teicoplanin Pseudoaglycone Derivative with Broad Antiviral Activity.Pharmaceutics · 2026Article
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- Changes in the chikungunya virus E1 glycoprotein domain II and hinge influence E2 conformation, infectivity, and virus-receptor interactions.Journal of virology · 2024Article
- Entry receptors - the gateway to alphavirus infection.The Journal of clinical investigation · 2023Review
- A bioactive phlebovirus-like envelope protein in a hookworm endogenous virus.Science advances · 2022Article
- Emerging Chikungunya Virus Variants at the E1-E1 Interglycoprotein Spike Interface Impact Virus Attachment and Inflammation.Journal of virology · 2022Article
- Cooperative Chikungunya Virus Membrane Fusion and Its Substoichiometric Inhibition by CHK-152 Antibody.Viruses · 2022Article
- Article
- The Structural Biology of Eastern Equine Encephalitis Virus, an Emerging Viral Threat.Pathogens (Basel, Switzerland) · 2021Review
- Resveratrol Inhibits Venezuelan Equine Encephalitis Virus Infection by Interfering with the AKT/GSK Pathway.Plants (Basel, Switzerland) · 2021Article
- Acidic pH-Induced Conformational Changes in Chikungunya Virus Fusion Protein E1: a Spring-Twisted Region in the Domain I-III Linker Acts as a Hinge Point for Swiveling Motion of Domains.Journal of virology · 2020Article
- A molecular understanding of alphavirus entry.PLoS pathogens · 2020Review
- Evolution-Driven Attenuation of Alphaviruses Highlights Key Glycoprotein Determinants Regulating Viral Infectivity and Dissemination.Cell reports · 2019Article
- Targeting domain-III hinging of dengue envelope (DENV-2) protein by MD simulations, docking and free energy calculations.Journal of molecular modeling · 2017Article
- Fusion of Enveloped Viruses in Endosomes.Traffic (Copenhagen, Denmark) · 2016Review
- Early Events in Chikungunya Virus Infection-From Virus Cell Binding to Membrane Fusion.Viruses · 2015Review
- Structural models of the membrane anchors of envelope glycoproteins E1 and E2 from pestiviruses.Virology · 2014Article
- Cross-inhibition of chikungunya virus fusion and infection by alphavirus E1 domain III proteins.Journal of virology · 2013Article
- Crystal structure of glycoprotein E2 from bovine viral diarrhea virus.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A key interaction between the alphavirus envelope proteins responsible for initial dimer dissociation during fusion.Journal of virology · 2013Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
The alphavirus Semliki Forest virus (SFV) infects cells through a low-pH-dependent membrane fusion reaction mediated by the virus fusion protein E1. Acidic pH initiates a series of E1 conformational changes that culminate in membrane fusion and include dissociation of the E1/E2 heterodimer, insertion of the E1 fusion loop into the target membrane, and refolding of E1 to a stable trimeric hairpin conformation. A highly conserved histidine (H3) on the E1 protein was previously shown to promote low-pH-dependent E1 refolding. An SFV mutant with an alanine substitution at this position (H3A) has a lower pH threshold and reduced efficiency of virus fusion and E1 trimer formation than wild-type SFV. Here we addressed the mechanism by which H3 promotes E1 refolding and membrane fusion. We identified E1 mutations that rescue the H3A defect. These revertants implicated a network of interactions that connect the domain I-domain III (DI-DIII) linker region with the E1 core trimer, including H3. In support of the importance of these interactions, mutation of residues in the network resulted in more acidic pH thresholds and reduced efficiencies of membrane fusion. In vitro studies of truncated E1 proteins demonstrated that the DI-DIII linker was required for production of a stable E1 core trimer on target membranes. Together, our results suggest a critical and previously unidentified role for the DI-DIII linker region during the low-pH-dependent refolding of E1 that drives membrane fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.