ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2011
Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Cellular crosstalk in the bone marrow niche.Journal of translational medicine · 2024Review
- Osteomacs promote maintenance of murine hematopoiesis through megakaryocyte-induced upregulation of Embigin and CD166.Stem cell reports · 2024Article
- The role of bone marrow microenvironment (BMM) cells in acute myeloid leukemia (AML) progression: immune checkpoints, metabolic checkpoints, and signaling pathways.Cell communication and signaling : CCS · 2023Review
- Neonatal Osteomacs and Bone Marrow Macrophages Differ in Phenotypic Marker Expression and Function.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- Leukemia stem cell-bone marrow microenvironment interplay in acute myeloid leukemia development.Experimental hematology & oncology · 2021Review
- Direct Reprogramming of Mouse Fibroblasts into Functional Osteoblasts.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2020Article
- Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis.Stem cells and development · 2018Article
- Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function.Blood advances · 2017Article
- A T Cell View of the Bone Marrow.Frontiers in immunology · 2016Article
- Activated leukocyte cell adhesion molecule (ALCAM or CD166) modulates bone phenotype and hematopoiesis.Journal of musculoskeletal & neuronal interactions · 2015Article
- The bone marrow endosteal niche: how far from the surface?Journal of cellular biochemistry · 2015Article
- Minireview: complexity of hematopoietic stem cell regulation in the bone marrow microenvironment.Molecular endocrinology (Baltimore, Md.) · 2014Review
- Article
- Cellular complexity of the bone marrow hematopoietic stem cell niche.Calcified tissue international · 2014Review
- Interactions between B lymphocytes and the osteoblast lineage in bone marrow.Calcified tissue international · 2013Review
- Pyk2 regulates megakaryocyte-induced increases in osteoblast number and bone formation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- Article
- Differential regulation of CXCL5 by FGF2 in osteoblastic and endothelial niche cells supports hematopoietic stem cell migration.Stem cells and development · 2012Article
- Absence of sclerostin adversely affects B-cell survival.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Osteoblasts (OBs) exert a prominent regulatory effect on hematopoietic stem cells (HSCs). We evaluated the difference in hematopoietic expansion and function in response to co-culture with OBs at various stages of development. Murine calvarial OBs were seeded directly (fresh) or cultured for 1, 2, or 3 weeks prior to seeding with 1000 Lin-Sca1 + cKit+ (LSK) cells for 1 week. Significant increases in the following hematopoietic parameters were detected when comparing co-cultures of fresh OBs to co-cultures containing OBs cultured for 1, 2, or 3 weeks: total hematopoietic cell number (up to a 3.4-fold increase), total colony forming unit (CFU) number in LSK progeny (up to an 18.1-fold increase), and percentage of Lin-Sca1+ cells (up to a 31.8-fold increase). Importantly, these studies were corroborated by in vivo reconstitution studies in which LSK cells maintained in fresh OB co-cultures supported a significantly higher level of chimerism than cells maintained in co-cultures containing 3-week OBs. Characterization of OBs cultured for 1, 2, or 3 weeks with real-time PCR and functional mineralization assays showed that OB maturation increased with culture duration but was not affected by the presence of LSK cells in culture. Linear regression analyses of multiple parameters measured in these studies show that fresh, most likely more immature OBs better promote hematopoietic expansion and function than cultured, presumably more mature OBs and suggest that the hematopoiesis-enhancing activity is mediated by cells present in fresh OB cultures de novo.
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Registered trials
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