Evidence map›Paper›PMID 21541754›Full record

ArticleParasitology research2011

Multiplex PCR identification of Taenia spp. in rodents and carnivores.

Mohammad N S Al-Sabi, Christian M O Kapel

Abstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Comprehensive Diagnosis, Treatment, and Outcome ofPathogens (Basel, Switzerland) · 2024
    Article
  3. Investigation and genetic polymorphism analysis of rodents infected withFrontiers in cellular and infection microbiology · 2024
    Article
  4. Identification and phylogenetic analysis ofInternational journal for parasitology. Parasites and wildlife · 2023
    Article
  5. Host and geographic differences in prevalence and diversity of gastrointestinal helminths of foxes (International journal for parasitology. Parasites and wildlife · 2021
    Article
  6. Article
  7. Article
  8. Molecular detection ofVeterinary world · 2017
    Article
  9. Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Mohammad N S Al-SabiDepartment of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark. moals@vet.dtu.dk
Christian M O Kapel
University of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genus Taenia includes several species of veterinary and public health importance, but diagnosis of the etiological agent in definitive and intermediate hosts often relies on labor intensive and few specific morphometric criteria, especially in immature worms and underdeveloped metacestodes. In the present study, a multiplex PCR, based on five primers targeting the 18S rDNA and ITS2 sequences, produced a species-specific banding patterns for a range of Taenia spp. Species typing by the multiplex PCR was compared to morphological identification and sequencing of cox1 and/or 12S rDNA genes. As compared to sequencing, the multiplex PCR identified 31 of 32 Taenia metacestodes from rodents, whereas only 14 cysts were specifically identified by morphology. Likewise, the multiplex PCR identified 108 of 130 adult worms, while only 57 were identified to species by morphology. The tested multiplex PCR system may potentially be used for studies of Taenia spp. transmitted between rodents and carnivores.

Indexed as

AnimalsCarnivoraDNA, HelminthDNA PrimersDNA, RibosomalDNA, Ribosomal SpacerMultiplex Polymerase Chain ReactionParasitologyRNA, RibosomalRNA, Ribosomal, 18SRodentiaTaeniaTaeniasisDNA, HelminthDNA PrimersDNA, RibosomalDNA, Ribosomal SpacerRNA, RibosomalRNA, ribosomal, 12SRNA, Ribosomal, 18S

Identifiers

PMID21541754
OpenAlexW1976522501

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.