ArticleJournal of industrial microbiology & biotechnology2011
Development of L-tryptophan production strains by defined genetic modification in Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 89 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analyses uncover novel genetic targets for enhanced l-tryptophan production inSynthetic and systems biotechnology · 2026Article
- Theranostic Probiotic Engineered Living Materials That Dynamically Respond to Inflammation Markers.Advanced healthcare materials · 2026Article
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan inInternational journal of molecular sciences · 2023Article
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- Adsorption Equilibria, Kinetics, and Column Dynamics of L-Tryptophan on Mixed-Mode Resin HD-1.ACS omega · 2022Article
- Advances and prospects in metabolic engineering of Escherichia coli for L-tryptophan production.World journal of microbiology & biotechnology · 2022Review
- Analyzing the genetic characteristics of a tryptophan-overproducing Escherichia coli.Bioprocess and biosystems engineering · 2021Article
- Biotechnological Production of the Sunscreen Pigment Scytonemin in Cyanobacteria: Progress and Strategy.Marine drugs · 2021Review
- Adsorption Separation of l-Tryptophan Based on the Hyper-Cross-Linked Resin XDA-200.ACS omega · 2021Article
- Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites.Nucleic acids research · 2021Article
- EngineeringSynthetic and systems biotechnology · 2020Article
- Construction of a switchable synthetic Escherichia coli for aromatic amino acids by a tunable switch.Journal of industrial microbiology & biotechnology · 2020Article
- Metabolic engineering for the production of l-phenylalanine in3 Biotech · 2019Review
- Metabolic engineering for improving L-tryptophan production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Review
- Rational design and analysis of an Escherichia coli strain for high-efficiency tryptophan production.Journal of industrial microbiology & biotechnology · 2018Article
- Phosphoenolpyruvate:glucose phosphotransferase system modification increases the conversion rate during L-tryptophan production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2017Article
- L-Tryptophan Production in Escherichia coli Improved by Weakening the Pta-AckA Pathway.PloS one · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Construction and improvement of industrial strains play a central role in the commercial development of microbial fermentation processes. L-tryptophan producers have usually been developed by classical random mutagenesis due to its complicated metabolic network and regulatory mechanism. However, in the present study, an L-tryptophan overproducing Escherichia coli strain was developed by defined genetic modification methodology. Feedback inhibitions of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (AroF) and anthranilate synthase (TrpED) were eliminated by site-directed mutagenesis. Expression of deregulated AroF and TrpED was achieved by using a temperature-inducible expression plasmid pSV. Transcriptional regulation of trp repressor was removed by deleting trpR. Pathway for L-Trp degradation was removed by deleting tnaA. L-phenylalanine and L-tyrosine biosynthesis pathways that compete with L-tryptophan biosynthesis were blocked by deleting their critical genes (pheA and tyrA). The final engineered E. coli can produce 13.3 g/l of L-tryptophan. Fermentation characteristics of the engineered strains were also analyzed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.