ArticleCurrent genetics2011
Distinguishing authentic mitochondrial and plastid DNAs from similar DNA sequences in the nucleus using the polymerase chain reaction.
Article in Current genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Rare maternal and biparental transmission of the cucumber mitochondrial DNA reveals sorting of polymorphisms among progenies.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Changes in DNA damage, molecular integrity, and copy number for plastid DNA and mitochondrial DNA during maize development.Journal of experimental botany · 2014Article
- Chloroplast DNA in mature and senescing leaves: a reappraisal.The Plant cell · 2014Article
- Differential methylation during maize leaf growth targets developmentally regulated genes.Plant physiology · 2014Article
- Article
- Effects of reduced chloroplast gene copy number on chloroplast gene expression in maize.Plant physiology · 2012Article
- Evolution of linear mitochondrial genomes in medusozoan cnidarians.Genome biology and evolution · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequences similar to those in the organellar genomes are also found in the nucleus. These non-coding sequences may be co-amplified by PCR with the authentic organellar DNA sequences, leading to erroneous conclusions. To avoid this problem, we describe an experimental procedure to prevent amplification of this "promiscuous" DNA when total tissue DNA is used with PCR. First, primers are designed for organelle-specific sequences using a bioinformatics method. These primers are then tested using methylation-sensitive PCR. The method is demonstrated for both end-point and real-time PCR with Zea mays, where most of the DNA sequences in the organellar genomes are also present in the nucleus. We use this procedure to quantify those nuclear DNA sequences that are near-perfect replicas of organellar DNA. This method should be useful for applications including phylogenetic analysis, organellar DNA quantification and clinical testing.
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Registered trials
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