ArticlePloS one2011
Proteomic analyses of nucleoid-associated proteins in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Molecular Kaleidoscope of Nucleoid-Associated Proteins Regulatory Network and Chromosome Architecture in Mycobacterium Tuberculosis.Current microbiology · 2026Review
- Phenotypic Characterization of Null Mutants of ΔcbpA and ΔcbpB in S. enterica Serovar Typhimurium.Current microbiology · 2025Article
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- Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization.Methods in molecular biology (Clifton, N.J.) · 2024Article
- NFrontiers in microbiology · 2024Article
- Histones with an unconventional DNA-binding mode in vitro are major chromatin constituents in the bacterium Bdellovibrio bacteriovorus.Nature microbiology · 2023Article
- Monoclonal Antibody Disrupts Biofilm Structure and Restores Antibiotic Susceptibility in an Orthopedic Implant Infection Model.Antibiotics (Basel, Switzerland) · 2023Article
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.Microbiology spectrum · 2023Article
- Growth temperature and chromatinization in archaea.Nature microbiology · 2022Article
- Effect of Environmental Temperatures on Proteome Composition of Salmonella enterica Serovar Typhimurium.Molecular & cellular proteomics : MCP · 2022Article
- ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment.Science advances · 2022Article
- Dps Is a Universally Conserved Dual-Action DNA-Binding and Ferritin Protein.Journal of bacteriology · 2022Review
- Relationship between the Chromosome Structural Dynamics and Gene Expression-A Chicken and Egg Dilemma?Microorganisms · 2022Review
- The horizontal transfer of Pseudomonas aeruginosa PA14 ICE PAPI-1 is controlled by a transcriptional triad between TprA, NdpA2 and MvaT.Nucleic acids research · 2021Article
- Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.Transcription · 2021Review
- HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanisms for Chromosome Segregation in Bacteria.Frontiers in microbiology · 2021Review
- Addressing the Possibility of a Histone-Like Code in Bacteria.Journal of proteome research · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe bacterial nucleoid contains several hundred kinds of nucleoid-associated proteins (NAPs), which play critical roles in genome functions such as transcription and replication. Several NAPs, such as Hu and H-NS in Escherichia coli, have so far been identified. METHODOLOGY/PRINCIPAL
findingsLog- and stationary-phase cells of E. coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were lysed in spermidine solutions. Nucleoids were collected by sucrose gradient centrifugation, and their protein constituents analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Over 200 proteins were identified in each species. Envelope and soluble protein fractions were also identified. By using these data sets, we obtained lists of contaminant-subtracted proteins enriched in the nucleoid fractions (csNAP lists). The lists do not cover all of the NAPs, but included Hu regardless of the growth phases and species. In addition, the csNAP lists of each species suggested that the bacterial nucleoid is equipped with the species-specific set of global regulators, oxidation-reduction enzymes, and fatty acid synthases. This implies bacteria individually developed nucleoid associated proteins toward obtaining similar characteristics. CONCLUSIONS/SIGNIFICANCE: Ours is the first study to reveal hundreds of NAPs in the bacterial nucleoid, and the obtained data set enabled us to overview some important features of the nucleoid. Several implications obtained from the present proteomic study may make it a landmark for the future functional and evolutionary study of the bacterial nucleoid.
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