ArticlePloS one2011
Linkage mapping and comparative genomics using next-generation RAD sequencing of a non-model organism.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
145 citing papers in PubMed, 297 citations in OpenAlex.
- Genetic diversity study and molecular marker development for economic traits in Korean pine based on RAD-Seq.Frontiers in plant science · 2026Article
- Genome-wide QTL and eQTL mapping reveal genes associated with growth rate trait of the Pacific white shrimp (Litopenaeus vannamei).BMC genomics · 2024Article
- Genetic diversity and population structure of Piper nigrum (black pepper) accessions based on next-generation SNP markers.PloS one · 2024Article
- Lepidopteran Synteny Units reveal deep chromosomal conservation in butterflies and moths.G3 (Bethesda, Md.) · 2023Article
- Additive and non-additive genetic variance in juvenile Sitka spruce (Tree genetics & genomes · 2023Article
- Silencing RNAs expressed from W-linkedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Recovering individual haplotypes and a contiguous genome assembly from pooled long-read sequencing of the diamondback moth (Lepidoptera: Plutellidae).G3 (Bethesda, Md.) · 2022Article
- Genome-Wide Population Structure and Selection Signatures of Yunling Goat Based on RAD-seq.Animals : an open access journal from MDPI · 2022Article
- Early stages of speciation with gene flow in the Amazilia Hummingbird (Ecology and evolution · 2022Article
- Assemblies of the genomes of parasitic wasps using meta-assembly and scaffolding with genetic linkage.G3 (Bethesda, Md.) · 2022Article
- Genome-Wide Association Mapping of Virulence Genes in Wheat Karnal Bunt FungusFrontiers in microbiology · 2022Article
- Response to selection for parasitism of a suboptimal, low-preference host in an aphid parasitoid.Evolutionary applications · 2021Article
- Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius).BMC genomics · 2021Article
- A combined RAD-Seq and WGS approach reveals the genomic basis of yellow color variation in bumble bee Bombus terrestris.Scientific reports · 2021Article
- Sex Chromosome Turnover in Bent-Toed Geckos (Genes · 2021Article
- A frog with three sex chromosomes that co-mingle together in nature: Xenopus tropicalis has a degenerate W and a Y that evolved from a Z chromosome.PLoS genetics · 2020Article
- Genome-wide analysis of diamondback moth, Plutella xylostella L., from Brassica crops and wild host plants reveals no genetic structure in Australia.Scientific reports · 2020Article
- Population structure of Apodemus flavicollis and comparison to Apodemus sylvaticus in northern Poland based on RAD-seq.BMC genomics · 2020Article
- Article
- Characterization of the First W-Specific Protein-Coding Gene for Sex Identification inFrontiers in genetics · 2020Article
85 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals. This approach has broad potential for genetic analysis of non-model organisms including genotype-phenotype association mapping, phylogeography, population genetics and scaffolding genome assemblies through linkage mapping. We constructed a RAD library using genomic DNA from a Plutella xylostella (diamondback moth) backcross that segregated for resistance to the insecticide spinosad. Sequencing of 24 individuals was performed on a single Illumina GAIIx lane (51 base paired-end reads). Taking advantage of the lack of crossing over in homologous chromosomes in female Lepidoptera, 3,177 maternally inherited RAD alleles were assigned to the 31 chromosomes, enabling identification of the spinosad resistance and W/Z sex chromosomes. Paired-end reads for each RAD allele were assembled into contigs and compared to the genome of Bombyx mori (n = 28) using BLAST, revealing 28 homologous matches plus 3 expected fusion/breakage events which account for the difference in chromosome number. A genome-wide linkage map (1292 cM) was inferred with 2,878 segregating RAD alleles inherited from the backcross father, producing chromosome and location specific sequenced RAD markers. Here we have used RAD sequencing to construct a genetic linkage map de novo for an organism that has no previous genome data. Comparative analysis of P. xyloxtella linkage groups with B. mori chromosomes shows for the first time, genetic synteny appears common beyond the Macrolepidoptera. RAD sequencing is a powerful system capable of rapidly generating chromosome specific data for non-model organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.