ArticleJournal of oral microbiology2011
Genomic comparison of invasive and rare non-invasive strains reveals Porphyromonas gingivalis genetic polymorphisms.
Article in Journal of oral microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
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- Contribution of -Omics Technologies in the Study ofInternational journal of molecular sciences · 2022Review
- Modulatory Mechanisms of Pathogenicity inMicroorganisms · 2022Review
- The RagA and RagB proteins of Porphyromonas gingivalis.Molecular oral microbiology · 2021Review
- Variability in Genomic and Virulent Properties ofFrontiers in cellular and infection microbiology · 2019Article
- Genomic repeats, misassembly and reannotation: a case study with long-read resequencing of Porphyromonas gingivalis reference strains.BMC genomics · 2018Article
- Article
- Structure of RagB, a major immunodominant outer-membrane surface receptor antigen of Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- Genome Sequence of Porphyromonas gingivalis Strain AJW4.Genome announcements · 2015Article
- Porphyromonas gingivalis RagB is a proinflammatory signal transducer and activator of transcription 4 agonist.Molecular oral microbiology · 2015Article
- Association of the invasion ability of Porphyromonas gingivalis with the severity of periodontitis.Virulence · 2015Article
- Invasion of Porphyromonas gingivalis strains into vascular cells and tissue.Journal of oral microbiology · 2015Article
- Identification and characterization of Porphyromonas gingivalis client proteins that bind to Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorphyromonas gingivalis strains are shown to invade human cells in vitro with different invasion efficiencies, varying by up to three orders of magnitude.
objectiveWe tested the hypothesis that invasion-associated interstrain genomic polymorphisms are present in P. gingivalis and that putative invasion-associated genes can contribute to P. gingivalis invasion.
designUsing an invasive (W83) and the only available non-invasive P. gingivalis strain (AJW4) and whole genome microarrays followed by two separate software tools, we carried out comparative genomic hybridization (CGH) analysis.
resultsWe identified 68 annotated and 51 hypothetical open reading frames (ORFs) that are polymorphic between these strains. Among these are surface proteins, lipoproteins, capsular polysaccharide biosynthesis enzymes, regulatory and immunoreactive proteins, integrases, and transposases often with abnormal GC content and clustered on the chromosome. Amplification of selected ORFs was used to validate the approach and the selection. Eleven clinical strains were investigated for the presence of selected ORFs. The putative invasion-associated ORFs were present in 10 of the isolates. The invasion ability of three isogenic mutants, carrying deletions in PG0185, PG0186, and PG0982 was tested. The PG0185 (ragA) and PG0186 (ragB) mutants had 5.1×10(3)-fold and 3.6×10(3)-fold decreased in vitro invasion ability, respectively.
conclusionThe annotation of divergent ORFs suggests deficiency in multiple genes as a basis for P. gingivalis non-invasive phenotype.
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