ArticleWorld journal of biological chemistry2010
Protective effects of anti-ricin A-chain antibodies delivered intracellularly against ricin-induced cytotoxicity.
Article in World journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Ricin and Ricinus communis in pharmacology and toxicology-from ancient use and "Papyrus Ebers" to modern perspectives and "poisonous plant of the year 2018".Naunyn-Schmiedeberg's archives of pharmacology · 2019Review
- Ricin Toxicity: Clinical and Molecular Aspects.Reports of biochemistry & molecular biology · 2016Article
- Modeling of toxin-antibody interaction and toxin transport toward the endoplasmic reticulum.Journal of biological physics · 2016Article
- Article
- Clinical and Pathological Findings Associated with Aerosol Exposure of Macaques to Ricin Toxin.Toxins · 2015Article
- Proteomic study of differential protein expression in mouse lung tissues after aerosolized ricin poisoning.International journal of molecular sciences · 2014Article
- Modelling effects of internalized antibody: a simple comparative study.Theoretical biology & medical modelling · 2014Article
- Conformation-dependent high-affinity potent ricin-neutralizing monoclonal antibodies.BioMed research international · 2013Article
- Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2012Article
- Humanization and characterization of an anti-ricin neutralization monoclonal antibody.PloS one · 2012Article
- A reaction-diffusion model of the receptor-toxin-antibody interaction.Theoretical biology & medical modelling · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
aimTo evaluate the ability of anti-ricin A-chain antibodies, delivered intracellularly, to protect against ricin-induced cytotoxicity in RAW264.7 cells.
methodsAnti-deglycosylated ricin A-chain antibody and RAC18 anti-ricin A-chain monoclonal antibody were delivered intracellularly by encapsulating in liposomes or via conjugation with the cell-penetrating MTS-transport peptide. RAW264.7 cells were incubated with these antibodies either before or after ricin exposure. The changes in cytotoxicity were estimated by MTT assay. Co-localization of internalized antibody and ricin was evaluated by fluorescence microscopy.
resultsInternalized antibodies significantly increased cell viability either before or after ricin exposure compared to the unconjugated antibodies. Fluorescence microscopy confirmed the co-localization of internalized antibodies and ricin inside the cells.
conclusionIntracellular delivery of antibodies to neutralize the ricin toxin after cellular uptake supports the potential use of cell-permeable antibodies for post-exposure treatment of ricin intoxication.
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Registered trials
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