ArticleEndocrinology2011
Visualization of estrogen receptor transcriptional activation in zebrafish.
Article in Endocrinology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 91 citations in OpenAlex.
- Article
- Zebrafish neuromast development: a target for endocrine disrupting chemicals?Frontiers in toxicology · 2026Article
- Hormone-induced thrombosis is mediated through non-canonical fibrin(ogen) aggregation and a novel estrogen target in zebrafish.bioRxiv : the preprint server for biology · 2024Article
- A novel ERβ high throughput microscopy platform for testing endocrine disrupting chemicals.Heliyon · 2024Article
- The Impaired Wound Healing Process Is a Major Factor in Remodeling of the Corneal Epithelium in Adult and Adolescent Patients With Keratoconus.Investigative ophthalmology & visual science · 2023Article
- Zebrafish as an emerging model to study estrogen receptors in neural development.Frontiers in endocrinology · 2023Review
- Estrogens regulate early embryonic development of the olfactory sensory system via estrogen-responsive glia.Development (Cambridge, England) · 2022Article
- Hyperactivation of Wnt/β-catenin and Jak/Stat3 pathways in human and zebrafish foetal growth restriction models: Implications for pharmacological rescue.Frontiers in cell and developmental biology · 2022Article
- Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development.Frontiers in pharmacology · 2022Review
- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Estrogen Acts Through Estrogen Receptor 2b to Regulate Hepatobiliary Fate During Vertebrate Development.Hepatology (Baltimore, Md.) · 2020Article
- Fluorescent Reporter Zebrafish Line for Estrogenic Compound Screening Generated Using a CRISPR/Cas9-Mediated Knock-in System.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Sexual Dimorphic Distribution of Hypothalamic Tachykinin1 Cells and Their Innervations to GnRH Neurons in the Zebrafish.Frontiers in endocrinology · 2020Article
- Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo.Toxicology and applied pharmacology · 2019Article
- Estrogen Activation of G-Protein-Coupled Estrogen Receptor 1 Regulates Phosphoinositide 3-Kinase and mTOR Signaling to Promote Liver Growth in Zebrafish and Proliferation of Human Hepatocytes.Gastroenterology · 2019Article
- Comparison the Effect of Ferutinin and 17β-Estradiol on Bone Mineralization of Developing Zebrafish (International journal of molecular sciences · 2019Article
- Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism.EBioMedicine · 2018Article
- Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.Scientific reports · 2018Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Neuroendocrine disruption in animal models due to exposure to bisphenol A analogues.Frontiers in neuroendocrinology · 2017Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Estrogens regulate a diverse range of physiological processes and affect multiple tissues. Estrogen receptors (ERs) regulate transcription by binding to DNA at conserved estrogen response elements, and such elements have been used to report ER activity in cultured cells and in transgenic mice. We generated stable, transgenic zebrafish containing five consecutive elements upstream of a c-fos minimal promoter and green fluorescent protein (GFP) to visualize and quantify transcriptional activation in live larvae. Transgenic larvae show robust, dose-dependent estrogen-dependent fluorescent labeling in the liver, consistent with er gene expression, whereas ER antagonists inhibit GFP expression. The nonestrogenic steroids dexamethasone and progesterone fail to activate GFP, confirming ER selectivity. Natural and synthetic estrogens activated the transgene with varying potency, and two chemicals, genistein and bisphenol A, preferentially induce GFP expression in the heart. In adult fish, fluorescence was observed in estrogenic tissues such as the liver, ovary, pituitary gland, and brain. Individual estrogen-responsive neurons and their projections were visualized in the adult brain, and GFP-positive neurons increased in number after 17β-estradiol exposure. The transgenic estrogen-responsive zebrafish allow ER signaling to be monitored visually and serve as in vivo sentinels for detection of estrogenic compounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.