ArticleThe Journal of biological chemistry2011
The acylation state of surface lipoproteins of mollicute Acholeplasma laidlawii.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- A comparative in silico analysis of the vlhA gene regions of Mycoplasma gallisepticum and Mycoplasma synoviae isolates from commercial hen farms in Mexico.Access microbiology · 2025Article
- Fatty acids ofMicrobiology spectrum · 2024Article
- Sequence analyses of a lipoprotein conserved with bacterial actins responsible for swimming motility of wall-less helicalmicroPublication biology · 2023Article
- Flow-Cytometric Method for Viability Analysis of Mycoplasma gallisepticum and Other Cell-Culture-Contaminant Mollicutes.Current microbiology · 2021Article
- Comparison of Current Methods for Signal Peptide Prediction in Phytoplasmas.Frontiers in microbiology · 2021Article
- Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry.Journal of visualized experiments : JoVE · 2018Article
- Identification of the Lyso-FormJournal of bacteriology · 2017Article
- Cosmid based mutagenesis causes genetic instability in Streptomyces coelicolor, as shown by targeting of the lipoprotein signal peptidase gene.Scientific reports · 2016Article
- Comparative genomic analysis of seven Mycoplasma hyosynoviae strains.MicrobiologyOpen · 2015Article
- Lipoproteins of slow-growing Mycobacteria carry three fatty acids and are N-acylated by apolipoprotein N-acyltransferase BCG_2070c.BMC microbiology · 2013Article
- N-acylation of lipoproteins: not when sour.Journal of bacteriology · 2012Article
- Environment-mediated accumulation of diacyl lipoproteins over their triacyl counterparts in Staphylococcus aureus.Journal of bacteriology · 2012Article
- Phosphatidylglycerol::prolipoprotein diacylglyceryl transferase (Lgt) of Escherichia coli has seven transmembrane segments, and its essential residues are embedded in the membrane.Journal of bacteriology · 2012Article
- Novel bacterial lipoprotein structures conserved in low-GC content gram-positive bacteria are recognized by Toll-like receptor 2.The Journal of biological chemistry · 2012Article
- A phylum level analysis reveals lipoprotein biosynthesis to be a fundamental property of bacteria.Protein & cell · 2012Review
- The iron-regulated staphylococcal lipoproteins.Frontiers in cellular and infection microbiology · 2012Review
- The ppm operon is essential for acylation and glycosylation of lipoproteins in Corynebacterium glutamicum.PloS one · 2012Article
- Complete genome and proteome of Acholeplasma laidlawii.Journal of bacteriology · 2011Article
- Arming the troops: Post-translational modification of extracellular bacterial proteins.Science progressReview
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acylation of the N-terminal Cys residue is an essential, ubiquitous, and uniquely bacterial posttranslational modification that allows anchoring of proteins to the lipid membrane. In gram-negative bacteria, acylation proceeds through three sequential steps requiring lipoprotein diacylglyceryltransferase, lipoprotein signal peptidase, and finally lipoprotein N-acyltransferase. The apparent lack of genes coding for recognizable homologs of lipoprotein N-acyltransferase in gram-positive bacteria and Mollicutes suggests that the final step of the protein acylation process may be absent in these organisms. In this work, we monitored the acylation state of eight major lipoproteins of the mollicute Acholeplasma laidlawii using a combination of standard two-dimensional gel electrophoresis protein separation, blotting to nitrocellulose membranes, and MALDI-MS identification of modified N-terminal tryptic peptides. We show that for each A. laidlawii lipoprotein studied a third fatty acid in an amide linkage on the N-terminal Cys residue is present, whereas diacylated species were not detected. The result thus proves that A. laidlawii encodes a lipoprotein N-acyltransferase activity. We hypothesize that N-acyltransferases encoded by genes non-homologous to N-acyltransferases of gram-negative bacteria are also present in other mollicutes and gram-positive bacteria.
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