ArticleProtein expression and purification2011
The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal.
Article in Protein expression and purification, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hHProtein science : a publication of the Protein Society · 2025Article
- Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Monitoring of enzymatic cleavage reaction of GST-fusion protein on biolayer interferometry sensor.Biophysics and physicobiology · 2024Article
- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The high-throughput production of membrane proteins.Emerging topics in life sciences · 2021Article
- N-Terminal Protein Labeling with N-Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein-Protein Interactions Using Labeled Protein.Current protocols · 2021Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyIIRussian journal of bioorganic chemistry · 2020Article
- Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells.Current microbiology · 2019Article
- High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives.Open biology · 2016Review
- Article
- A Novel and Fast Purification Method for Nucleoside Transporters.Frontiers in molecular biosciences · 2016Article
- Expression and purification of the alpha subunit of the epithelial sodium channel, ENaC.Protein expression and purification · 2016Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains.Protein science : a publication of the Protein Society · 2014Article
- Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system.Frontiers in microbiology · 2014Review
- The pCri System: a vector collection for recombinant protein expression and purification.PloS one · 2014Article
- Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox.Journal of biotechnology · 2013Article
- Research applications of proteolytic enzymes in molecular biology.Biomolecules · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recombinant proteins typically include one or more affinity tags to facilitate purification and/or detection. Expression constructs with affinity tags often include an engineered protease site for tag removal. Like other enzymes, the activities of proteases can be affected by buffer conditions. The buffers used for integral membrane proteins contain detergents, which are required to maintain protein solubility. We examined the detergent sensitivity of six commonly-used proteases (enterokinase, factor Xa, human rhinovirus 3C protease, SUMOstar, tobacco etch virus protease, and thrombin) by use of a panel of 94 individual detergents. Thrombin activity was insensitive to the entire panel of detergents, thus suggesting it as the optimal choice for use with membrane proteins. Enterokinase and factor Xa were only affected by a small number of detergents, making them good choices as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.