ArticleProceedings of the National Academy of Sciences of the United States of America1990
Immunocytology on microwave-fixed cells reveals rapid and agonist-specific changes in subcellular accumulation patterns for cAMP or cGMP.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
- cGMP Signaling in Arterioles Revealed by FRET-Based Real-Time Measurement in Mice In Vivo.Acta physiologica (Oxford, England) · 2026Article
- A Layered View on Focal Adhesions.Biology · 2021Review
- Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.Scientific reports · 2019Article
- cGMP Signaling in the Cardiovascular System-The Role of Compartmentation and Its Live Cell Imaging.International journal of molecular sciences · 2018Review
- Fixation strategies for retinal immunohistochemistry.Progress in retinal and eye research · 2015Review
- Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein kinase A anchoring.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The brain chondroitin sulfate proteoglycan brevican associates with astrocytes ensheathing cerebellar glomeruli and inhibits neurite outgrowth from granule neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Identification and characterization of the type-IVA cyclic AMP-specific phosphodiesterase RD1 as a membrane-bound protein expressed in cerebellum.The Biochemical journal · 1995Article
- A-kinase anchoring proteins: a key to selective activation of cAMP-responsive events?Molecular and cellular biochemistry · 1993Review
- Adenylate cyclase-mediated vascular responses of rabbit aorta, mesenteric artery and skin microcirculation.British journal of pharmacology · 1993Article
- Role of phosphodiesterases in the regulation of endothelial permeability in vitro.The Journal of clinical investigation · 1993Article
- Rapid effects of insulin on cyclic GMP location in an intact protozoan.Experientia · 1992Article
- Phorbol ester induced osteoclast-like differentiation of a novel human leukemic cell line (FLG 29.1).The Journal of cell biology · 1992Article
- Influence of selective phosphodiesterase inhibitors on human neutrophil functions and levels of cAMP and Cai.Naunyn-Schmiedeberg's archives of pharmacology · 1991Article
- Intracellular distribution of microinjected antisense oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Rapid accumulation of cyclic GMP near activated vitamin D receptors.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Oxygen radicals induce human endothelial cells to express GMP-140 and bind neutrophils.The Journal of cell biology · 1991Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a method for cAMP and cGMP immunocytology based upon fixation by microwave irradiation. Fixation by microwave irradiation prevented three problems found with other fixation methods: nucleotide loss from cells, nucleotide diffusion within cells, and chemical modification of immunologic epitopes. Six agonists (four that stimulate adenylate cyclase and two that stimulate guanylate cyclase) produced cAMP or cGMP accumulation patterns that were agonist-specific, dose-dependent, detectable at physiologic concentrations of hormone, and time-dependent within 15 sec to 30 min. cAMP accumulation after 1 mM forskolin was greatest in the nucleus. Isoproterenol, prostaglandin E2, or calcitonin caused initial accumulation of cAMP along the plasma membrane, but later accumulation was greater in the cytoplasm. With calcitonin the later accumulation of cAMP was selectively perinuclear and along the nuclear membrane. Sodium nitroprusside stimulated cGMP accumulation diffusely throughout the cytoplasm. Atrial natriuretic peptide initiated cGMP accumulation near the plasma membrane, and cGMP accumulation moved from there into the cytoplasm. In conclusion, microwave irradiation preserved cell structure and allowed visualization of expected as well as unsuspected changes in intracellular accumulation patterns of cAMP and cGMP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.