ArticleBiotechnology and bioengineering2011
Microprinted feeder cells guide embryonic stem cell fate.
Article in Biotechnology and bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The role of cytosolic mitochondrial DNA as a mitochondrial danger signal for stromal fibroblasts in forming the tumor-promoting microenvironment following radiation exposure.Journal of radiation research · 2026Review
- Stem cell colony interspacing effect on differentiation to neural cells.Journal of tissue engineering and regenerative medicine · 2018Article
- Statistical analysis of multi-dimensional, temporal gene expression of stem cells to elucidate colony size-dependent neural differentiation.Molecular omics · 2018Article
- Biomaterials-Based Approaches to Tumor Spheroid and Organoid Modeling.Advanced healthcare materials · 2018Review
- Microprinted Stem Cell Niches Reveal Compounding Effect of Colony Size on Stromal Cells-Mediated Neural Differentiation.Advanced healthcare materials · 2018Article
- Efficient feeder cells preparation system for large-scale preparation and application of induced pluripotent stem cells.Scientific reports · 2017Article
- Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Molecular Analysis of Stromal Cells-Induced Neural Differentiation of Mouse Embryonic Stem Cells.PloS one · 2016Article
- Concise Review: Stem Cell Microenvironment on a Chip: Current Technologies for Tissue Engineering and Stem Cell Biology.Stem cells translational medicine · 2015Review
- Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system.Scientific reports · 2015Article
- Robotic production of cancer cell spheroids with an aqueous two-phase system for drug testing.Journal of visualized experiments : JoVE · 2015Article
- High Throughput, Polymeric Aqueous Two-Phase Printing of Tumor Spheroids.Advanced functional materials · 2014Article
- Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-Cancer Drug Testing in 3D Culture.Cellular and molecular bioengineering · 2014Article
- Hierarchical hydrodynamic flow confinement: efficient use and retrieval of chemicals for microscale chemistry on surfaces.Langmuir : the ACS journal of surfaces and colloids · 2014Article
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- Cell co-culture patterning using aqueous two-phase systems.Journal of visualized experiments : JoVE · 2013Article
- Aqueous biphasic microprinting approach to tissue engineering.Biomicrofluidics · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We introduce a non-contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are printed on a substrate residing within a bath of the immersion aqueous phase. Due to their affinity to the denser phase, cells remain localized within the drops and adhere to regions of the substrate underneath the drops. We show the utility of this technology for creating duplex heterocellular stem cell niches by printing two different support cell types on a gel surface and overlaying them with mouse embryonic stem cells (mESCs). As desired, the type of printed support cell spatially direct the fate of overlaid mESCs. Interestingly, we found that interspaced mESCs colonies on differentiation-inducing feeder cells show enhanced neuronal differentiation and give rise to dense networks of neurons. This cell printing technology provides unprecedented capabilities to efficiently identify the role of various feeder cells in guiding the fate of stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.