ArticleJournal of microbiology (Seoul, Korea)2011
Development of Porphyromonas gingivalis-specific quantitative real-time PCR primers based on the nucleotide sequence of rpoB.
Article in Journal of microbiology (Seoul, Korea), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Host insulin resistance caused byFrontiers in cellular and infection microbiology · 2023Pooled it
- Characteristics of the Salivary Microbiota in Periodontal Diseases and Potential Roles of Individual Bacterial Species To Predict the Severity of Periodontal Disease.Microbiology spectrum · 2023Article
- Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis.Microorganisms · 2021Article
- Article
- Periodontal inflamed surface area as a novel numerical variable describing periodontal conditions.Journal of periodontal & implant science · 2017Article
- In Vitro Effect of Porphyromonas gingivalis Methionine Gamma Lyase on Biofilm Composition and Oral Inflammatory Response.PloS one · 2016Article
- Viable Compositional Analysis of an Eleven Species Oral Polymicrobial Biofilm.Frontiers in microbiology · 2016Article
- Reducing the bioactivity of Tannerella forsythia lipopolysaccharide by Porphyromonas gingivalis.Journal of microbiology (Seoul, Korea) · 2014Article
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies.
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Registered trials
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