ArticleParasitology research2011
Isolation and identification of Legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with PCR.
Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 32 citations in OpenAlex.
- Review
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- Free-living amoebae and their associated bacteria in Austrian cooling towers: a 1-year routine screening.Parasitology research · 2016Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
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- Distribution of Acanthamoeba Genotypes Isolated from Recreational and Therapeutic Geothermal Water Sources in Southwestern Iran.Environmental health insights · 2016Article
- Identification of Paenibacillus as a Symbiont in Acanthamoeba.Current microbiology · 2015Article
- Assessment of Legionella pneumophila in recreational spring water with quantitative PCR (Taqman) assay.Pathogens and global health · 2015Article
- Surveillance of parasitic Legionella in surface waters by using immunomagnetic separation and amoebae enrichment.Pathogens and global health · 2015Article
- Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking water.Parasitology research · 2014Article
- Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay.Environmental science and pollution research international · 2013Article
- The role of water in healthcare-associated infections.Current opinion in infectious diseases · 2013Review
- Differential Legionella spp. survival between intracellular and extracellular forms in thermal spring environments.Environmental science and pollution research international · 2013Article
- Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay.Parasitology research · 2013Article
- Real-time PCR method for the detection and quantification of Acanthamoeba species in various types of water samples.Parasitology research · 2013Article
- Diversity and seasonal impact of Acanthamoeba species in a subtropical rivershed.BioMed research international · 2013Article
- Molecular characterization of Acanthamoeba isolated in water treatment plants and comparison with clinical isolates.Parasitology research · 2012Article
- Occurrence of Thermotolerant Hartmannella vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran.Iranian journal of parasitology · 2012Article
- Morphological and Molecular Survey of Naegleria spp. in Water Bodies Used for Recreational Purposes in Rasht city, Northern Iran.Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella were detected with the direct DNA extraction method, Legionella culture method, and free-living amoebae (FLA) culture method from weak alkaline carbonate spring water in Taiwan. Moreover, we also investigated the existence of Acanthamoeba, Hartmannella, and Naegleria, ubiquitous FLA in aquatic environments, to identify the correlations between existing Legionella. This study reports detecting Legionella in 15 of the 51 weak alkaline carbonate spring water samples (29.4%). This work also found five of the 51 samples (9.8%) analyzed by the direct DNA extraction method, three of the 51 (5.9%) samples analyzed by the Legionella culture method, and 11 of the 51 samples (21.6%) evaluated using the FLA culture method to be positive for Legionella. The most frequently identified Legionella species was the Legionella-like amoebal pathogen (n=5), followed by unidentified Legionella spp. (n=4), and Legionella pneumophila (n=4), Legionella fairfieldensis (n=3), and then Legionella rubrilucens (n=2). Legionella waltersii was detected once. The occurrence of Acanthamoeba, Hartmannella, and Naegleria were 5.9% (3/51), 52.9% (27/51), and 5.9% (3/51), respectively. All Hartmannella isolates were identified as Hartmannella vermiformis, and Naegleria isolates were all identified as Naegleria australiensis. The three Acanthamoeba isolates were identified as one Acanthamoeba polyphaga and two Acanthamoeba jacobsi. H. vermiformis (40.7%) were Legionella hosts, including all of the amoebae-resistant Legionella detected in the present study. Therefore, the important correlations between Legionella and H. vermiformis require further clarification. The combined results of this survey confirm that Legionella and FLA are ubiquitous in weak alkaline carbonate spring water in Taiwan.
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