ArticleJournal of nuclear medicine : official publication, Society of Nuclear Medicine2011
A beta-camera integrated with a microfluidic chip for radioassays based on real-time imaging of glycolysis in small cell populations.
Article in Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Development of a Lensless Radiomicroscope for Cellular-Resolution Radionuclide Imaging.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2023Article
- Microfluidics-Coupled Radioluminescence Microscopy forAnalytical chemistry · 2021Article
- In silico optimization of radioluminescence microscopy.Journal of biophotonics · 2018Article
- Automatic concentration and reformulation of PET tracers via microfluidic membrane distillation.Lab on a chip · 2017Article
- Performance evaluation ofMedical physics · 2017Article
- On chip two-photon metabolic imaging for drug toxicity testing.Biomicrofluidics · 2017Article
- Accelerated Biofluid Filling in Complex Microfluidic Networks by Vacuum-Pressure Accelerated Movement (V-PAM).Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Imaging metabolic heterogeneity in cancer.Molecular cancer · 2016Review
- Quantitative assessments of glycolysis from single cells.Technology · 2015Article
- Compact microfluidic device for rapid concentration of PET tracers.Lab on a chip · 2014Article
- Rational development of radiopharmaceuticals for HIV-1.Nuclear medicine and biology · 2014Review
- Optimization of microfluidic PET tracer synthesis with Cerenkov imaging.The Analyst · 2013Article
- High-resolution radioluminescence microscopy of 18F-FDG uptake by reconstructing the β-ionization track.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2013Article
- Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2013Article
- Betabox: a beta particle imaging system based on a position sensitive avalanche photodiode.Physics in medicine and biology · 2013Article
- Dysfunctional oxidative phosphorylation makes malignant melanoma cells addicted to glycolysis driven by the (V600E)BRAF oncogene.Oncotarget · 2013Article
- Batch-reactor microfluidic device: first human use of a microfluidically produced PET radiotracer.Lab on a chip · 2013Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
unlabelledAn integrated β-camera and microfluidic chip was developed that is capable of quantitative imaging of glycolysis radioassays using (18)F-FDG in small cell populations down to a single cell. This paper demonstrates that the integrated system enables digital control and quantitative measurements of glycolysis in B-Raf(V600E)-mutated melanoma cell lines in response to specific B-Raf inhibition.
methodsThe β-camera uses a position-sensitive avalanche photodiode to detect charged particle-emitting probes within a microfluidic chip. The integrated β-camera and microfluidic chip system was calibrated, and the linearity was measured using 4 different melanoma cell lines (M257, M202, M233, and M229). Microfluidic radioassays were performed with cell populations ranging from hundreds of cells down to a single cell. The M229 cell line has a homozygous B-Raf(V600E) mutation and is highly sensitive to a B-Raf inhibitor, PLX4032. A microfluidic radioassay was performed over the course of 3 days to assess the cytotoxicity of PLX4032 on cellular (18)F-FDG uptake.
resultsThe β-camera is capable of imaging radioactive uptake of (18)F-FDG in microfluidic chips. (18)F-FDG uptake for a single cell was measured using a radioactivity concentration of 37 MBq/mL during the radiotracer incubation period. For in vitro cytotoxicity monitoring, the β-camera showed that exposure to 1 μM PLX4032 for 3 days decreased the (18)F-FDG uptake per cell in highly sensitive M229 cells, compared with vehicle controls.
conclusionThe integrated β-camera and microfluidic chip can provide digital control of live cell cultures and allow in vitro quantitative radioassays for multiple samples simultaneously.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.